Role of Fks1p and Matrix Glucan in Candida albicans Biofilm Resistance to an Echinocandin, Pyrimidine, and Polyene
Role of Fks1p and Matrix Glucan in Candida albicans Biofilm Resistance to an Echinocandin, Pyrimidine, and Polyene
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DOI：
10.1128/aac.00227-10
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发表时间：
2010-08-01
影响因子：
4.9
通讯作者：
Andes, David R.
中科院分区：
文献类型：
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作者：
Nett, Jeniel E.;Crawford, Kyler;Andes, David R.
念珠菌感染通常涉及器械表面的耐药生物膜生长。葡聚糖合成酶基因FKS 1与念珠菌生物膜中的三唑耐药性有关。我们测试了FKS 1调节对其他抗真菌药物敏感性的影响。FKS 1表达的减少使生物膜对阿替霉素B、阿尼芬净和氟胞嘧啶更敏感。观察到过表达FKS 1的生物膜对阿尼芬净和阿替霉素B的耐药性增加。这些发现表明念珠菌生物膜葡聚糖螯合是一种多药耐药机制。
Candida infections frequently involve drug-resistant biofilm growth on device surfaces. Glucan synthase gene FKS1 has been linked to triazole resistance in Candida biofilms. We tested the impact of FKS1 modulation on susceptibility to additional antifungal classes. Reduction of FKS1 expression rendered biofilms more susceptible to amphotericin B, anidulafungin, and flucytosine. Increased resistance to anidulafungin and amphotericin B was observed for biofilms overexpressing FKS1. These findings suggest that Candida biofilm glucan sequestration is a multidrug resistance mechanism.