Assessing identity, phenotype, and fate of endothelial progenitor cells.
Assessing identity, phenotype, and fate of endothelial progenitor cells.
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评估内皮祖细胞的身份、表型和命运。
DOI：
10.1161/atvbaha.107.155960
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Yoder MC
中科院分区：
文献类型：
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作者：
Hirschi KK;Ingram DA;Yoder MC
Asahara 在 al10 文章中，通过体外和体内测定将成人外周血中的人 CD34 表达细胞（CD34 表达富集 15.7%）作为推定的 EPC 进行研究。在体外确定假定的 EPC 的一些独特方面时，Asahara 等人 10 报道称，细胞粘附在纤连蛋白包被的培养皿上的频率高于 1 型胶原蛋白包被的培养皿，并显示出纺锤形形态。有趣的是，当与 CD34 耗尽的单核细胞共培养时，假定的 CD34 EPC 会在中心形成圆形细胞簇，并在外围形成纺锤形细胞的芽。贴壁的假定 EPC 表达多种通常由人脐静脉内皮细胞表达的细胞表面蛋白，并且这些标记物的表达在体外随着时间的推移而增加。进一步的研究提供了证据，证明这些假定的 EPC（CD34 或血管内皮生长因子受体 2 [Flk-1] 细胞富集至 20%）在体内注射到诱导后肢缺血的裸鼠体内时定位于新生血管形成区域。因此，Asahara 等人在一篇文章中提出了循环 EPC 的概念、EPC 行为的体外观察、推定 EPC 向血管损伤部位的体内迁移以及出生后血管发生的范例。
the Asahara at al10 article, human CD34 expressing cells (15.7% enriched for CD34 expression) in adult peripheral blood were interrogated as putative EPCs via in vitro and in vivo assays. While determining some of the unique aspects of putative EPCs in vitro, Asahara et al10 reported that the cells adhered to fibronectin-coated dishes with greater frequency than to type 1 collagen coated dishes and displayed a spindle-shaped morphology. Of interest, the putative CD34 EPCs when cocultured with CD34 depleted mononuclear cells formed clusters of round cells centrally and sprouts of spindle-shaped cells at the periphery. The adherent putative EPCs expressed a variety of cell surface proteins typically expressed by human umbilical vein endothelial cells and expression of these markers increased over time in vitro. Further studies provided evidence of these putative EPCs (CD34 or vascular endothelial growth factor receptor 2 [Flk-1] cells enriched to 20%) localizing to areas of neovascularization when injected in vivo into nude mice with induced hindlimb ischemia. Thus, in one article Asahara et al10 brought forth concepts of circulating EPCs, in vitro observations of EPC behavior, in vivo migration of putative EPCs to sites of vascular injury, and the paradigm of postnatal vasculogenesis.