The endocannabinoid system in the brain of Carassius auratus and its possible role in the control of food intake
The endocannabinoid system in the brain of Carassius auratus and its possible role in the control of food intake
复制标题
鲫鱼大脑中的内源性大麻素系统及其在控制食物摄入中的可能作用
作者：
M. Valenti;E. Cottone;R. Martínez;N. Pedro;M. Rubio;Maria;M. Franzoni;M. Delgado;V. Marzo
大麻素受体和内源性大麻素花生四烯酸和2-花生四烯酸甘油被认为可以调节几种动物门的食物摄入。哺乳动物大麻素CB 1和CB 2受体的直系同源物已在鱼类中鉴定。我们研究了这种内源性大麻素系统在金鱼鲫鱼大脑中的存在及其在食物消耗中的作用。CB 1样免疫反应性分布在整个金鱼脑中。前脑在端脑和下丘脑后叶的下叶中显示出强烈的CB 1样免疫反应性。内源性大麻素在C. 24小时的食物剥夺伴随着花生四烯酸的显著增加，但不包括2-花生四烯酸甘油，仅在端脑中的水平。 花生四烯酸酰胺在腹膜内给药后2小时内对摄食量产生剂量依赖性影响，分别在低剂量（1 pg/g体重）或中等剂量（10 pg/g）下显著增加或减少摄食量，最高试验剂量（100 pg/g）无活性。 我们认为，内源性大麻素可能会不同程度地有助于适应鱼类食物短缺的反应。
Cannabinoid receptors and the endocannabinoids anandamide and 2‐arachidonoylglycerol have been suggested to regulate food intake in several animal phyla. Orthologs of the mammalian cannabinoid CB1 and CB2 receptors have been identified in fish. We investigated the presence of this endocannabinoid system in the brain of the goldfish Carassius auratus and its role in food consumption. CB1‐like immunoreactivity was distributed throughout the goldfish brain. The prosencephalon showed strong CB1‐like immunoreactivity in the telencephalon and the inferior lobes of the posterior hypothalamus. Endocannabinoids were detected in all brain regions of C. auratus and an anandamide‐hydrolysing enzymatic activity with features similar to those of mammalian fatty acid amide hydrolase was found. Food deprivation for 24 h was accompanied by a significant increase of anandamide, but not 2‐arachidonoylglycerol, levels only in the telencephalon. Anandamide caused a dose‐dependent effect on food intake within 2 h of intraperitoneal administration to satiated fish and significantly enhanced or reduced food intake at low (1 pg/g body weight) or intermediate (10 pg/g) doses, respectively, the highest dose tested (100 pg/g) being inactive. We suggest that endocannabinoids might variously contribute to adaptive responses to food shortage in fish.
影响因子：
56.9
作者：
DEVANE, WA;HANUS, L;MECHOULAM, R
通讯作者：
MECHOULAM, R
水性铅暴露会影响雄性黑头呆鱼 (Pimephales promelas) 的大脑内源性大麻素含量，但不会影响雌性黑头呆鱼 (Pimephales promelas)。
影响因子：
3.4
作者：
Rademacher,DavidJ;Weber,DanielN;Hillard,CeciliaJ
通讯作者：
Hillard,CeciliaJ