Model system for the in vitro testing of a synthetic histidine peptide against Candida species grown directly on the denture surface of patients with denture stomatitis.
Model system for the in vitro testing of a synthetic histidine peptide against Candida species grown directly on the denture surface of patients with denture stomatitis.
复制标题
用于体外测试合成组氨酸肽针对直接生长在义齿口腔炎患者义齿表面上的念珠菌的模型系统。
DOI：
10.1016/0022-3913(88)90353-8
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Gwinnett,AJ
中科院分区：
文献类型：
--
作者：
Santarpia3rd,RP;Renner,RP;Pollock,JJ;Gwinnett,AJ
义齿表面为微生物菌种的生长提供了一个病灶，这些微生物菌种的作用是引发、加重和维持临床疾病。本研究描述了一个模型系统的开发，用于测试试剂对这些微生物物种栖息在义齿表面的有效性。通过体外生长模式观察到，该模型允许检测微生物植物群的代表性样品。聚L-组氨酸对白色念珠菌和念珠菌均有抑制作用。glabrata从假牙表面生长到营养肉汤。对照组和治疗组义齿盘的扫描电子显微镜显示，聚-L-组氨酸已经从义齿表面消除了大多数微生物植物群，或者已经影响了仍然存在于表面上的嗜盐芽孢杆菌的明显变形。从微生物学研究来看，聚-L-组氨酸似乎对仍然附着的扭曲芽生孢子造成了直接但非致命的损害，因为后者仍然能够促进无试剂肉汤中的生长。聚-L-组氨酸的抗真菌作用被观察到依赖于多肽的浓度。所有患者获得的数据是一致的，无论他们的义齿性口炎分类。
The denture surface provides a nidus for the growth of microbial species that act to initiate, aggravate, and maintain clinical disease. The present investigation describes the development of a model system for the testing of the effectiveness of agents against these microbial species inhabiting the denture surface. It was observed through in vitro growth patterns that the model permitted the testing of representive samples of the microbial flora. Poly-L-histidine was observed to inhibit bothCandida albicansandC. glabratafrom growing from the denture surface into nutrient broth. Scanning electron microscopy of control and treated denture disks revealed that poly-L-histidine had either eliminated most microbial flora from the denture surface or had effected a noticeable distortion of thoseCandidablastospores still present on the surface. From microbiologic studies, it appeared that poly-L-histidine had inflicted direct but not lethal damage to the still-attached distorted blastospores because the latter were still able to promote growth in agent-free broth. The antifungal effects of poly-L-histidine were observed to be dependent on the concentration of the polypeptide. The data obtained were consistent for all of the patients regardless of their denture stomatitis classification.