Coupling between Flagellar Motor Rotation and Proton Flux in Bacteria
Coupling between Flagellar Motor Rotation and Proton Flux in Bacteria
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细菌中鞭毛运动旋转与质子通量之间的耦合
DOI：
10.1143/jpsj.52.4019
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发表时间：
1983
影响因子：
1.7
通讯作者：
S. Hayashi
中科院分区：
文献类型：
--
作者：
F. Oosawa;S. Hayashi
细菌鞭毛马达根据跨膜的电化学电势梯度由质子流驱动。为了理解质子的电势能和化学势能到机械功的有效和平滑的转换，质子流和马达的旋转之间假定为松耦合。在提出的机制中，排列在圆盘和圆盘周围的环上的两种蛋白质分子重复由质子结合调节的快速且可逆的反应。根据这一机理，导出了电动机产生的转矩和质子通量与电动机转速的关系。数值计算的结果与大多数的实验结果显示出合理的协议。特别注意的是自由能的逆变换的可能性。预计通过外力使电机反向旋转，质子通量将减小。
The bacterial flagellar motor is driven by a flow of proton according to the electrochemical potential gradient across the membrane. For understanding efficient and smooth transformation of both electric and chemical potential energies of proton into a mechanical work, loose coupling has been assumed between the flow of protons and the rotation of the motor. In the mechanism proposed, two kinds of protein molecules arranged on a disc and on a ring surrounding the disc repeat fast and reversible reactions regulated by the binding of protons. Based on this mechanism, the relations of the torque generated in the motor and the flux of protons to the speed of rotation of the motor have been derived. The results of numerical calculations show reasonable agreement with most of the experimental results. Particular attention has been paid to the possibility of reverse transformation of free energy. It is expected that by rotating the motor in the reverse direction by an external force, the flux of protons is decre...