MECHANOSENSITIVITY OF NMDA RECEPTORS IN CULTURED MOUSE CENTRAL NEURONS
MECHANOSENSITIVITY OF NMDA RECEPTORS IN CULTURED MOUSE CENTRAL NEURONS
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DOI：
10.1016/0896-6273(94)90032-9
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发表时间：
1994-09-01
期刊：
影响因子：
16.2
通讯作者：
ASCHER, P
中科院分区：
文献类型：
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作者：
PAOLETTI, P;ASCHER, P
渗透压和静水压力的变化被发现调制了不同膜片钳配置的培养的小鼠胚胎神经元的NMDA反应。在有核斑块中，NMDA电流因外渗浓度的降低而增强，而在高渗溶液中则降低。对于红藻氨酸、甘氨酸或GABA的反应，这些变化在低浓度的NMDA时更为明显。它们可以通过直接改变有核、外向外、内向外和细胞附着的贴片中的吸管压力来模拟。在全细胞模式下也观察到NMDA反应的渗透敏感性，但只有在延长透析时间后才能观察到。NMDA受体的机械敏感性可能在经历膜张力变化的神经元区域发挥重要作用，如棘突或生长锥体。
Changes in osmotic and hydrostatic pressure were found to modulate NMDA responses of cultured embryonic mouse neurons recorded in various patch-clamp configurations. In nucleated patches, NMDA currents were potentiated by reductions in external osmolarity and were reduced in hyper-osmotic solutions. These changes, which were greater for low concentrations of NMDA, were not observed for responses to kainate, glycine, or GABA. They could be mimicked by directly changing the pipette pressure in nucleated, outside-out, inside-out, and cell-attached patches. Osmosensitivity of NMDA responses was also observed in the whole-cell mode, but only after prolonged dialysis. Mechanosensitivity of NMDA receptors could have an important role in neuronal regions experiencing changes in membrane tension, such as spines or growth cones.