Low-level laser therapy for zymosan-induced arthritis in rats: Importance of illumination time
Low-level laser therapy for zymosan-induced arthritis in rats: Importance of illumination time
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DOI：
10.1002/lsm.20516
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发表时间：
2007-07-01
影响因子：
2.4
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
--
作者：
Castano, Ana P.;Dai, Tianhong;Hamblin, Michael R.
背景：多年来，人们一直认为低水平激光（或光）疗法（LLLT）可以改善与各种形式关节炎相关的疼痛、肿胀和炎症。光被认为被线粒体发色团吸收，导致三磷酸腺苷（ATP）、活性氧和/或环AMP的产生增加，并通过转录因子的激活导致基因转录。然而，尽管有许多关于LLLT在关节炎和一般医学中的积极作用的报道，它的使用仍然存在争议。对于所有适应症（包括关节炎），最佳的光学参数很难确定，迄今为止尚不清楚。方法：对大鼠膝关节注射酶生诱导炎症性关节炎进行LLLT试验。我们比较了由高通量和低通量（3和30 J/cm 2）组成的照明方案，在高和低辐照度（5和50 mlV/cm(2)）下每天使用8个10 nm激光，持续5天，与常规皮质类固醇（地塞米松）治疗的阳性对照。结果：810 nm激光照射在消肿方面非常有效（几乎与地塞米松一样好），较长的照射时间（10或100分钟相比于1分钟）在确定有效性方面比传递的总通量或辐照度更重要。LLLT诱导的关节肿胀减轻与炎症标志物血清前列腺素E2 （PGE2）的降低相关。结论：810nm激光LLLT治疗炎症性关节炎有较好的疗效。无论总影响或辐照度如何，较长的照明时间比短时间更有效。这些数据将对设计LLLT治疗各种关节炎的临床试验有价值。
Background: It has been proposed for many years that low-level laser (or light) therapy (LLLT) can ameliorate the pain, swelling, and inflammation associated with various forms of arthritis. Light is thought to be absorbed by mitochondrial chromophores leading to an increase in adenosine triphosphate (ATP), reactive oxygen species and/or cyclic AMP production and consequent gene transcription via activation of transcription factors. However, despite many reports about the positive effects of LLLT in arthritis and in medicine in general, its use remains controversial. For all indications (including arthritis) the optimum optical parameters have been difficult to establish and so far are unknown.Methods: We tested LLLT on rats that had zymosan injected into their knee joints to induce inflammatory arthritis. We compared illumination regimens consisting of a high and low fluence (3 and 30 J/cm 2), delivered at high and low irradiance (5 and 50 mlV/cm(2)) using 8 10-nm laser light daily for 5 days, with the positive control of conventional corticosteroid (dexamethasone) therapy.Results: Illumination with 810-nm laser was highly effective (almost as good as dexamethasone) at reducing swelling and a longer illumination time (10 or 100 minutes compared to 1 minute) was more important in determining effectiveness than either the total fluence delivered or the irradiance. LLLT induced reduction of joint swelling correlated with reduction in the inflammatory marker serum prostaglandin E2 (PGE2).Conclusion: LLLT with 810-nm laser is highly effective in treating inflammatory arthritis in this model. Longer illumination times were more effective than short times regardless of total fluence or irradiance. These data will be of value in designing clinical trials of LLLT for various arthritides.