Comparative study of poly (lactic-co-glycolic acid)/tricalcium phosphate scaffolds incorporated or coated with osteogenic growth factors for enhancement of bone regeneration
Comparative study of poly (lactic-co-glycolic acid)/tricalcium phosphate scaffolds incorporated or coated with osteogenic growth factors for enhancement of bone regeneration
复制标题
掺入或涂覆成骨生长因子的聚（乳酸-乙醇酸）/磷酸三钙支架增强骨再生的比较研究
DOI：
10.1016/j.jot.2014.01.002
复制
发表时间：
2014-04-01
影响因子：
6.6
通讯作者：
Qin, Ling
中科院分区：
文献类型：
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作者：
Chen, Shi-hui;Zheng, Li-zhen;Qin, Ling
骨移植替代品通常用于治疗大型骨缺损，特别是如果它们还可以作为局部给药系统，能够促进骨再生。本研究采用低温快速成型技术制备了聚乳酸-乙醇酸共聚物(PLGA)和磷酸三钙(TCP)复合支架。为了优化给药系统，开发了两种不同的方法来加载植物大分子淫羊藿苷(ICT)或骨形态发生蛋白-2(BMP-2)进行体内疗效研究。一种是在制造过程中将生长因子加入支架中的“掺入法”，而另一种是将制成的支架浸入含有生长因子的制备溶液中的“涂层法”。含有这些生长因子的支架被命名为P/T/ICT和P/T/BMP-2，而涂有这些生长因子的支架分别被命名为P/T+ICT和P/T+BMP-2。以未加任何载荷的粉末冶金支架作为对照。在兔尺骨骨缺损模型上比较了这些支架的骨再生效果。移植后的骨再生和血管生成通过高分辨率外周定量CT和磁共振成像进行评估。P/T/ICT支架成骨效果较好，与对照组相比提高了83.8%，与P/T/BMP-2相比提高了72.0%。尽管如预期的那样，P/T+BMP-2支架显示出最好的整体骨再生，但含有ICT的P/T支架被证明是一种创新的、具有成本效益的生物活性支架，它也显著促进了骨再生，具有在骨科应用中得到验证的潜力。版权所有(C)2014，作者。爱思唯尔(新加坡)私人有限公司出版。
Bone graft substitutes are commonly used to treat large bone defects, particularly if they can additionally act as a local delivery system for therapeutic agents capable of enhancing bone regeneration. In this study, composite scaffolds made of poly (lactic-co-glycolic acid) (PLGA) and tricalcium phosphate (TCP) called P/T were fabricated by a low-temperature rapid prototyping technique. In order to optimise the delivery system, two different approaches for loading either the phytomolecule icaritin (ICT) or bone morphogenetic protein-2 (BMP-2) were developed for an in vivo efficacy study. One was an "incorporating approach" in which the growth factor was incorporated into the scaffold during fabrication, whereas the other was a "coating approach" in which the fabricated scaffold was immersed into a preparative solution containing the growth factor. Scaffolds incorporating these growth factors were termed P/T/ICT and P/T/BMP-2, while scaffolds that had these growth factors coated on to them were named, respectively, P/T + ICT and P/T + BMP-2. A P/T scaffold without any loading was used as the control. The bone regeneration effect of these scaffolds was compared in an ulnar bone defect model in rabbits. Bone regeneration and angiogenesis was evaluated by high-resolution peripheral quantitative computed tomography and magnetic resonance imaging postimplantation. Bone regeneration was better with the P/T/ICT scaffolds with an 83.8% improvement compared with the control, and a 72.0% improvement compared with the P/T/BMP-2 treatment. Although the P/T + BMP-2 scaffold demonstrated, as expected, the best overall bone regeneration, the P/T scaffold with incorporated ICT was shown to be an innovative and cost-effective bioactive scaffold which also significantly enhanced bone regeneration with the potential to be validated for orthopaedic applications. Copyright (C) 2014, The Authors. Published by Elsevier (Singapore) Pte Ltd.