A novel means of identifying the neuraminidase type of currently circulating human A(H1) influenza viruses
A novel means of identifying the neuraminidase type of currently circulating human A(H1) influenza viruses
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DOI：
10.1016/j.virusres.2004.02.017
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发表时间：
2004-07-01
期刊：
影响因子：
5
通讯作者：
Hampson, AW
中科院分区：
文献类型：
--
作者：
Hurt, AC;Barr, IG;Hampson, AW
随着最近出现和传播的流感A（H1N2）病毒似乎是由循环的A（H1N1）和A（H3N2）毒株的重配引起的，在流行病学研究中需要确定神经氨酸酶类型，以区分流感A（H1N2）和A（H1N1）毒株。已开发用于筛选流感病毒对神经氨酸酶抑制剂药物的潜在耐药性的基于荧光的神经氨酸酶抑制试验似乎适用于此目的。当与神经氨酸酶抑制剂扎那米韦一起使用时，该测定能够为N1或N2型神经氨酸酶的鉴定提供93.5%的阳性预测值。该检测方法能够以低成本筛选大量甲型流感病毒，以确定人群中传播的甲型H1N2或甲型H1N1病毒的相对水平。(C)2004 Elsevier B.V.保留所有权利。
With the recent emergence and spread of influenza A(H1N2) viruses which appear to have arisen by reassortment of circulating A(H1N1) and A(H3N2) strains, there is a need in epidemiological studies to determine the neuraminidase type in order to differentiate between influenza A(H1N2) and A(H1N1) strains. A fluorescence-based neuraminidase enzyme inhibition assay that has been developed to screen influenza viruses for potential resistance to the neuraminidase inhibitor drugs appears to be suitable for this purpose. When used with the neuraminidase inhibitor zanamivir the assay was able to provide a positive predictive value of 93.5% for the identification of neuraminidase type N1 or N2. This assay enables a large number of influenza A viruses to be screened at low cost to determine relative levels of A(H1N2) or A(H1N1) viruses circulating in the population. (C) 2004 Elsevier B.V. All rights reserved.