Pomegranate peel polyphenols alleviate insulin resistance through the promotion of insulin signaling pathway in skeletal muscle of metabolic syndrome rats
Pomegranate peel polyphenols alleviate insulin resistance through the promotion of insulin signaling pathway in skeletal muscle of metabolic syndrome rats
复制标题
石榴皮多酚通过促进代谢综合征大鼠骨骼肌胰岛素信号通路减轻胰岛素抵抗
DOI：
10.1016/j.fshw.2022.03.034
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发表时间：
2022-07-01
影响因子：
7
通讯作者：
Li，Jianke
中科院分区：
文献类型：
--
作者：
Zhang，Xitong;Du，Lin;Li，Jianke
胰岛素抵抗（IR）被认为是代谢综合征（MetS）的重要致病因素。本研究探讨石榴皮多酚（PPPs）是否可以防止代谢综合征的发展，通过改善IR大鼠。采用高脂饲料（HFD）诱导雄性SD大鼠代谢综合征（MetS），并补充不同剂量的PPPs，共12周。结果表明，HFD诱导的胰岛素抵抗大鼠血糖、血脂代谢紊乱，肌纤维形态学异常，PPPs 300 mg/kg·d治疗可明显逆转这些不良影响。此外，PPPs处理显著增加胰岛素抵抗大鼠骨骼肌组织中胰岛素受体（InsR）和磷酸化胰岛素受体底物1（IRS-1）的蛋白表达，刺激过氧化物酶体增殖物激活受体γ（PPARγ）和磷酸肌醇3-激酶（PI 3 K）/蛋白激酶B（AKT/PKB）信号通路，磷酸化糖原合成酶激酶-3 β（GSK-3β）和葡萄糖转运蛋白4（GLUT 4）的蛋白水平升高。结果提示PPPs可能通过增强胰岛素敏感性和调节糖代谢来减轻IR。
Insulin resistance (IR) has been considered to be an important causative factor of metabolic syndrome (MetS). The present study investigated whether pomegranate peel polyphenols (PPPs) could prevent the development of MetS by improving IR in rats. Male Sprague-Dawley (SD) rats were fed high fat diet (HFD) to induce MetS and supplemented with different dosages of PPPs for 12 weeks. The results showed that HFD-induced insulin resistant rats had disordered metabolism of blood glucose, blood lipid, and terrible muscle fiber morphology when compared with normal diet-fed rats, but PPPs treatment at a dosage of 300 mg/kg·day significantly reversed these negative effects. Moreover, in skeletal muscle tissue of insulin resistant rats, PPPs treatments significantly increased the protein expressions of insulin receptor (InsR) and phosphorylated insulin receptor substrate 1 (IRS-1), stimulated peroxisome proliferator activated receptor gamma (PPARγ) and phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT/PKB) signaling pathway, and aggrandized the protein levels of phosphorylated glycogen synthase kinase-3β (GSK-3β) and glucose transporter 4 (GLUT4). Our results suggest that PPPs possess of the beneficial effects on alleviating IR by enhancing insulin sensitivity and regulating glucose metabolism.