Application of a Molecular Method for the Identification of a Gigaspora margarita Isolate Released in a Field
Application of a Molecular Method for the Identification of a Gigaspora margarita Isolate Released in a Field
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应用分子方法鉴定田间释放的玛格丽塔大孢菌分离物
DOI：
10.1111/j.1747-0765.2005.tb00015.x
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发表时间：
2005
影响因子：
2
通讯作者：
T. Marumoto
中科院分区：
文献类型：
--
作者：
K. Yokoyama;T. Tateishi;M. Saito;T. Marumoto
A molecular technique for the identification of the Gigaspora margarita isolate CK based on the detection of a DNA sequence of 235 bp as its diagnostic marker was evaluated to investigate the survival and establishment of introduced arbuscular mycorrizal fungi (AMF) in a field ecosystem. In March 2001, roots and rhizosphere soil of Eragrostis curvula and Miscanthus sinensis were collected from the Mizunashi River at Mt. Fugendake (Nagasaki Prefecture, Japan), where plant seeds and AMF including G. margarita CK had been introduced for reforestation after the occurrence of repeated pyroclastic flows. We detected the marker sequence from DNA preparations of E. curvula roots and Gigaspora spores in the rhizosphere. This clearly showed that the isolate occurred at both hyphal and sporal stages. It was shown that the isolate survived and developed a life cycle in the revegetation area for 4 years. It was confirmed that the method was effective for tracing the isolate in samples collected from field ecosystems.
富士山东南坡原生演替火山荒漠中优势植物丛枝菌根定殖
DOI：
--
发表时间：
2004
期刊：
Mycorrhiza 14
影响因子：
--
作者：
B.Wu;K.Isobe;R.Ishii
通讯作者：
R.Ishii