Human fat cell adenylate cyclase: regional differences in adrenaline responsiveness
Human fat cell adenylate cyclase: regional differences in adrenaline responsiveness
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人类脂肪细胞腺苷酸环化酶：肾上腺素反应性的区域差异
DOI：
10.1111/j.1365-2362.1977.tb01657.x
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发表时间：
1977
影响因子：
5.5
通讯作者：
G. Schlierf
中科院分区：
文献类型：
--
作者：
H. Kather;K. Zöllig;B. Simon;G. Schlierf
抽象的。本文研究了肾上腺素对人腹部和臀部脂肪组织膜上腺苷环化酶活力的影响。腹部组织的基础酶活性平均为1.0nmolcAMP/mg蛋白/15min，臀部幽灵的cAMP/mg蛋白活性平均为0.68nmolcAMP/mg蛋白/15min。最大有效浓度的氟化钠(20 mmoL/L)诱导两种膜制剂增加8倍。腹部组织幽灵对肾上腺素(1.0 mmol/L)的最大反应率平均为170%，臀膜为60%。产生半最大刺激所需的肾上腺素浓度在两个脑区的膜制剂中相似(约5×10-5mol/L)。
Abstract. The effect of adrenaline on adenylate cyclase activity in membranes prepared from human abdominal and gluteal adipose tissue was examined. Basal enzyme activity averaged 1.0 nmol cAMP/mg protein/15 min in abdominal tissue and 0.68 nmol cAMP/mg protein/15 min in ghosts from the buttock. Maximally effective concentrations of sodium fluoride (20 mmol/l) induced an eight‐fold increase in both membrane preparations. The maximal response to adrenaline (1.0 mmol/l) averaged 170% in ghosts of abdominal tissue compared with 60% in gluteal membranes. The concentrations of adrenaline required to produce half‐maximal stimulation were similar in membrane preparations of both regions (about 5 × 10‐5 mol/l).