The genetics of plant metabolism
The genetics of plant metabolism
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DOI：
10.1038/ng1815
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发表时间：
2006-07-01
期刊：
影响因子：
30.8
通讯作者：
Koornneef, Maarten
中科院分区：
文献类型：
--
作者：
Keurentjes, Joost J. B.;Fu, Jingyuan;Koornneef, Maarten
植物体内的代谢物组成和含量经常发生变异。然而，人们对这种变异在多大程度上具有遗传基础知之甚少。在这里，我们描述了在拟南芥的代谢物组成的自然变异的遗传分析。而不是专注于特定的代谢物，我们已经应用经验非靶向代谢组学，使用液相色谱-飞行时间质谱（LC-QTOF MS）。这揭示了A. thaliana加入在所有分析的14份材料中，仅检测到13.4%的质量峰。在来自两个最具分歧的种质的重组自交系（RIL）群体中检测到超过2，000个质量峰的数量性状基因座（QTL）分析，使得能够鉴定约75%的质量信号的QTL。超过三分之一的信号没有检测到任何一个父母，表明通过经典育种的代谢组成的修改的巨大潜力。
Variation for metabolite composition and content is often observed in plants. However, it is poorly understood to what extent this variation has a genetic basis. Here, we describe the genetic analysis of natural variation in the metabolite composition in Arabidopsis thaliana. Instead of focusing on specific metabolites, we have applied empirical untargeted metabolomics using liquid chromatography-time of flight mass spectrometry (LC-QTOF MS). This uncovered many qualitative and quantitative differences in metabolite accumulation between A. thaliana accessions. Only 13.4% of the mass peaks were detected in all 14 accessions analyzed. Quantitative trait locus (QTL) analysis of more than 2,000 mass peaks, detected in a recombinant inbred line (RIL) population derived from the two most divergent accessions, enabled the identification of QTLs for about 75% of the mass signals. More than one-third of the signals were not detected in either parent, indicating the large potential for modification of metabolic composition through classical breeding.