Characteristics and association with disease of two major subclones of Shiga toxin (Verocytotoxin)-producing strains of Escherichia coli (STEC) O157 that are present among isolates from patients in Germany
Characteristics and association with disease of two major subclones of Shiga toxin (Verocytotoxin)-producing strains of Escherichia coli (STEC) O157 that are present among isolates from patients in Germany
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DOI：
10.1016/s0732-8893(02)00474-1
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发表时间：
2002-12-01
影响因子：
2.9
通讯作者：
Smith, HR
中科院分区：
文献类型：
--
作者：
Beutin, L;Kaulfuss, S;Smith, HR
从居住在德国不同地区的168名患者中分离到产志贺氏毒素(Vercytotoxx)的大肠杆菌(STEC)O157。大多数分离株来自零星病例，并确认了7起STEC O157小规模暴发。对168株菌株进行了表型和基因特征检测，以确定德国发生的STEC O157的主要类型。噬菌体分型(PT)显示，PT8(n=54)和PT2(n=48)菌株最常见(60.7%)。STEC O157携带STX(2)基因与溶血性尿毒症综合征(100%)和血性腹泻(61.7%)密切相关。STX(2)基因在PT88、PT47(100%)、PT2(91.5%)和PT4(87.5%)菌株中出现频率较高，在其他PTS菌株中较少(33.3%)。PT8和PT2菌株形成了两个不同的群体，它们的活力、STX基因类型和它们引起的疾病的严重程度都不同。PT2和PT8菌株的脉冲场凝胶电泳和XbaI酶切的DNA与STX(1)和STX(2)特异性基因探针杂交显示，属于同一PT的流行病学无关菌株之间具有相似性。结果表明，STEC O157、PT2和PT8菌株形成两个不同的亚系，在德国和其他欧洲国家占主导地位。(C)2002 Elsevier Science Inc.保留所有权利。
Shiga toxin (Verocytotoxin) producing E. coli (STEC) O157 were isolated from 168 patients living in different parts of Germany. Most isolates were from sporadic cases and seven small outbreaks with STEC O157 were identified. The 168 strains were examined for phenotypic and genotypical traits in order to identify major types of STEC O157 occurring in Germany. Phage typing (PT) revealed PT8 (n = 54) and PT2 (n = 48) strains as most frequent (60.7%) among the isolates. Carriage of the stx(2) gene by STEC O157 was closely associated with hemolytic uremic syndrome (100%) and with bloody diarrhea (61.7%). The stx(2) gene was frequent in PT88, PT47 (both 100%), PT2 (91.5%) and PT4 (87.5%) strains and more rarely (33.3%) found in strains belonging to the other PTs. PT8 and PT2 strains formed two groups which differed from each other in their motility, stx-genotypes and the severity of the illness they caused. Pulsed-field gel electrophoresis of PT2 and PT8 strains and hybridization of XbaI digested DNA with stx(1) and stx(2) specific gene probes revealed similarities among epidemiologically unrelated strains belonging to the same PT. The results indicate that STEC O157 PT2 and PT8 strains form two distinct subelones which are dominating in Germany and other European countries. (C) 2002 Elsevier Science Inc. All rights reserved.