Rapid high-performance liquid chromatographic method for the determination of peroxyacetic acid
Rapid high-performance liquid chromatographic method for the determination of peroxyacetic acid
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DOI：
10.1016/0021-9673(95)01346-6
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发表时间：
1996-04-12
影响因子：
4.1
通讯作者：
Karst, U
中科院分区：
文献类型：
--
作者：
Pinkernell, U;Effkemann, S;Karst, U
过氧乙酸选择性氧化甲基对甲苯基硫醚（MTS）生成相应的亚砜（MTSO），然后快速分离硫化物和亚砜，这是在过氧化氢存在下快速、可靠测定该氧化剂的HPLC方法的基础。色谱分离所需的时间减少到小于1分钟。为了提高亚砜溶液的长期稳定性，二氧化锰催化分解过氧化氢。即使在存在10倍摩尔过量的过氧化氢的情况下，也实现了至少20小时的可储存性，而MTSO浓度没有显著增加。可以使用稳定的商用MTSO进行外部校准。对啤酒厂现场清洗消毒过程中的真实的样品进行了分析，并与经典的两步滴定法进行了比较。
The selective oxidation of methyl p-tolyl sulfide (MTS) to the corresponding sulfoxide (MTSO) by peroxyacetic acid and the subsequent rapid separation of the sulfide and sulfoxide are the basis for a fast and reliable HPLC method for the determination of this oxidizing agent in the presence of hydrogen peroxide. The time required for chromatographic separation was reduced to less than 1 min. To improve the long-term stability of the sulfoxide solution, hydrogen peroxide was decomposed catalytically by manganese dioxide. Even in the presence of a tenfold molar excess of hydrogen peroxide, a storability of at least 20 h without a significant increase in MTSO concentration was achieved. External calibration can be performed using the stable and commercially available MTSO. Real samples from a brewery cleaning-in-place disinfection process were analysed and the results were compared with those of the classical two-step titration.