SnoVectors for nuclear expression of RNA.
SnoVectors for nuclear expression of RNA.
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用于 RNA 核表达的 SnoVectors
DOI：
10.1093/nar/gku1050
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发表时间：
2015-01
影响因子：
14.9
通讯作者：
Chen LL
中科院分区：
文献类型：
--
作者：
Yin QF;Hu SB;Xu YF;Yang L;Carmichael GG;Chen LL
许多长链非编码rna （lncrna）被限制在细胞核内以发挥其功能。然而，设计用于表达mrna的常用载体尚未针对核rna的研究进行优化。我们最近报道了sno- lncrna不被封帽或聚腺苷化，而是在每一端被snorna及其相关蛋白终止。这些rna由内含子加工而成，严格限制在细胞核内。在这里，我们使用这些特征来设计表达向量，可以稳定地表达几乎任何感兴趣的序列，并将其积累限制在细胞核内。此外，这些rna似乎保留了正常的核关联和功能。在研究核RNA功能或需要避免输出到细胞质的情况下，snovator应该是有用的。
Many long noncoding RNAs (lncRNAs) are constrained to the nucleus to exert their functions. However, commonly used vectors that were designed to express mRNAs have not been optimized for the study of nuclear RNAs. We reported recently that sno-lncRNAs are not capped or polyadenylated but rather are terminated on each end by snoRNAs and their associated proteins. These RNAs are processed from introns and are strictly confined to the nucleus. Here we have used these features to design expression vectors that can stably express virtually any sequence of interest and constrain its accumulation to the nucleus. Further, these RNAs appear to retain normal nuclear associations and function. SnoVectors should be useful in conditions where nuclear RNA function is studied or where export to the cytoplasm needs to be avoided.
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