[Deletion of the env region in HERV-H-X gene and its expression in colon cancer].
[Deletion of the env region in HERV-H-X gene and its expression in colon cancer].
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发表时间：
2007-09
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通讯作者：
Qiao-yi Liang;Ze-feng Xu;R. Xu;Shu Zheng;Jia-yi Ding
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作者：
Qiao-yi Liang;Ze-feng Xu;R. Xu;Shu Zheng;Jia-yi Ding
背景与目的我们最近从结肠癌组织中发现了一个新基因HERV-H-X。本研究分析HERV-H-X的env区缺失情况，比较其与其他HERV-H前病毒env区开放阅读框（ORF）的表达情况，并研究其在结肠癌和正常结肠组织中的差异表达。方法对HERV-H-X和其他3种含完整env ORF的HERV-H前病毒（HERV-H/env62、HERV-H/env60和HERV-H/env59）进行多重比对。应用HERV-H-X特异性聚合酶链反应（PCR）和env ORF通用PCR方法检测HERV-H/env62、env60和env59在8对结肠癌组织及癌旁正常组织中的表达。应用实时荧光定量PCR方法检测17例结肠癌组织及癌旁正常组织中HERV-H-X的表达差异。结果HERV-H-X的env区缺失部分与HERV-H/env62、HERV-H/env60和HERV-H/env59的env ORF对应。HERV-H-X仅在结肠癌组织中检测到，而env ORF在癌组织和正常组织中都检测到。HERV-H-X在结肠癌组织中的表达是正常组织的24.9倍（P <0.01）。结论HERV-H-X在结肠癌组织中高表达，但其表达上调与env基因无关，因为其缺失env ORF。
BACKGROUND & OBJECTIVE We have identified a novel gene HERV-H-X from colon cancer tissue recently. This research was to analyze the deletion of the env region of HERV-H-X, to compare its expression with that of the env open reading frames (ORFs) in other HERV-H proviruses, and to study its differential expression in colon cancer and normal colon tissues. METHODS Multiple alignments were performed for HERV-H-X and 3 other HERV-H proviruses (HERV-H/env62, HERV-H/env60, and HERV-H/env59) containing intact env ORF. HERV-H-X-specific polymerase chain reaction (PCR) and env ORF-common PCR for HERV-H/env62, env60 and env59 were conducted to study their expression in 8 pairs of colon cancer and adjacent normal tissues. Real-time quantitative PCR was conducted to study the differential expression of HERV-H-X in 17 pairs of colon cancer and adjacent normal tissues. RESULTS The missing part in the env region of HERV-H-X corresponded to env ORFs in HERV-H/env62, HERV-H/env60 and HERV-H/env59. HERV-H-X was only detected in colon cancer tissues, while env ORFs were detected both in cancer and normal tissues. The expression of HERV-H-X was up-regulated in colon cancer tissues by 24.9 folds of that in normal tissues (P<0.01). CONCLUSION HERV-H-X is highly expressed in colon cancer, but its up-regulation is not related to env gene as it lacks an env ORF.