In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa
In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa
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DOI：
10.1016/j.jep.2004.02.011
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发表时间：
2004-06-01
影响因子：
5.4
通讯作者：
Folb, PI
中科院分区：
文献类型：
--
作者：
Clarkson, C;Maharaj, VJ;Folb, PI
疟疾流行率和分布的增加归因于若干因素，其中之一是抗药性寄生虫的出现和传播。目前正在努力发现和开发化学性质多样化的新的抗疟剂。本研究报告在体外抗疟原虫活性的134种植物类群原生或归化在南非，代表54个家庭，这是半定量选择使用加权标准。使用寄生虫乳酸脱氢酶（pLDH）测定法测试植物提取物对恶性疟原虫菌株D10的体外活性。在134种检测的物种中，49%显示出有希望的抗疟原虫活性（IC 50小于或等于10 μ g/ml），而17%被发现具有高活性（IC 50小于或等于5 μ g/ml）。一些植物种属首次被证明具有体外抗疟原虫活性。这些结果支持了一个合理的，而不是随机的方法来选择抗疟原虫筛选候选人，并确定了一些有前途的类群作为植物为基础的抗疟药作进一步调查。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
The increasing prevalence and distribution of malaria has been attributed to a number of factors, one of them being the emergence and spread of drug resistant parasites. Efforts are now being directed towards the discovery and development of new chemically diverse antimalarial agents. The present study reports on the in vitro antiplasmodial activity of 134 plant taxa native to or naturalised in South Africa, representing 54 families, which were selected semi-quantitatively using weighted criteria. The plant extracts were tested for in vitro activity against a Plasmodium falciparum strain D10 using the parasite lactate dehydrogenase (pLDH) assay. Of the 134 species assayed, 49% showed promising antiplasmodial activity (IC50 less than or equal to 10 mug/ml), while 17% were found to be highly active (IC50 less than or equal to 5 mug/ml). Several plant species and genera were shown for the first time to possess in vitro antiplasmodial activity. These results support a rational rather than random approach to the selection of antiplasmodial screening candidates, and identify a number of promising taxa for further investigation as plant-based antimalarial agents. (C) 2004 Elsevier Ireland Ltd. All rights reserved.