Intracellular Assembly of Nuclear-Targeted Gold Nanosphere Enables Selective Plasmonic Photothermal Therapy of Cancer by Shifting Their Absorption Wavelength toward Near-Infrared Region
Intracellular Assembly of Nuclear-Targeted Gold Nanosphere Enables Selective Plasmonic Photothermal Therapy of Cancer by Shifting Their Absorption Wavelength toward Near-Infrared Region
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DOI：
10.1021/acs.bioconjchem.7b00427
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发表时间：
2017-09-01
影响因子：
4.7
通讯作者：
El-Sayed, Mostafa A.
中科院分区：
文献类型：
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作者：
Panikkanvalappil, Sajanlal R.;Hooshmand, Nasrin;El-Sayed, Mostafa A.
尽管近红外(NIR)吸收纳米材料在等离子体光热治疗(PPT)中有着重要的应用，但它们在主动和被动靶向肿瘤治疗策略中的高产率合成和非特异性加热仍然具有挑战性。在本工作中，我们系统地证明了由于等离子体吸收带向近红外区域的显著移动，典型的非近红外吸收等离子体纳米粒子在细胞核区的原位聚集可以将它们转化为等离子体光热治疗中的有效的近红外光吸收剂。我们评估了核靶向AuNSs在内吞作用不同阶段作为光吸收剂的潜力，因为它们在细胞核区域的固有原位组装能力被认为是细胞内最不耐热的结构之一，在对健康细胞最小的损害的情况下选择性地破坏癌细胞。各种等离子体纳米粒子，如棒和立方体，已被用来阐明等离子体场耦合在组装纳米粒子中的作用及其随后的杀灭效率。利用离散偶极近似(DDA)技术进一步从实验和理论上证明了聚集态AuNSs的近红外吸收能力，这与等离子体光热治疗研究中观察到的结果是一致的。虽然目前的工作能够证明非近红外吸收的等离子体纳米颗粒通过在细胞核区域诱导局部等离子体加热作为等离子体光热疗法的潜在替代方案的实用性，但这些发现可能会在开发更有效的纳米颗粒用于有效的癌症治疗方式方面开辟新的可能性。
Despite the important applications of near-infrared (NIR) absorbing nanomaterials in plasmonic photothermal therapy (PPT), their high yield synthesis and nonspecific heating during the active- and passive-targeted cancer therapeutic strategies remain challenging. In the present work, we systematically demonstrate that in situ aggregation of typical non-NIR absorbing plasmonic nanoparticles at the nuclear region of the cells could translate them into an effective NIR photoabsorber in plasmonic photothermal therapy of cancer due to a significant shift of the plasmonic absorption band to the NIR region. We evaluated the potential of nuclear targeted AuNSs as photoabsorber at various stages of endocytosis by virtue of their inherent in situ assembling capabilities at the nuclear region of the cells, which has been considered as one of the most thermolabile structures within the cells, to selectively destruct cancer cells with minimal damage to healthy cells. Various plasmonic nanoparticles such as rods and cubes have been exploited to elucidate the role of plasmonic field coupling in assembled nanoparticles and their subsequent killing efficiency. The NIR absorbing capabilities of aggregated AuNSs have been further demonstrated both experimentally and theoretically using discrete dipolar approximation (DDA) techniques, which was in concordance with the observed results in plasmonic photothermal therapeutic studies. While the current work was able to demonstrate the utility of non-NIR absorbing plasmonic nanoparticles as a potential alternative for plasmonic photothermal therapy by inducing localized plasmonic heating at the nuclear region of the cells, these findings could potentially open up new possibilities in developing more efficient nanoparticles for efficient cancer treatment modalities.