Isolation and characterization of equine amnion mesenchymal stem cells.
Isolation and characterization of equine amnion mesenchymal stem cells.
复制标题
DOI：
10.1042/cbr20110004
复制
发表时间：
2011-09-13
期刊：
影响因子：
--
通讯作者：
Passeri S
中科院分区：
文献类型：
--
作者：
Coli A;Nocchi F;Lamanna R;Iorio M;Lapi S;Urciuoli P;Scatena F;Giannessi E;Stornelli MR;Passeri S
羊膜是一种特殊的组织，其细胞显示出多能干细胞的特征，被提出用于细胞治疗和再生医学。从马驹出生后收集的马羊膜中，我们分离出了来自中胚层的MSC（间充质干细胞），即EAMSC（马羊膜间充质干细胞）。细胞在α-MEM（α-改良最低必需培养基）中生长，并评价EGF（表皮生长因子）补充的效果。为了评估EAMSC的生长动力学，我们考虑了一些参数[PD（群体倍增）、倍数增加和DT（倍增时间）]。已报道了软骨形成、脂肪形成和成骨类型的细胞的分化及其通过细胞荧光研究的表位表达。EGF补充的培养基导致PD生长参数和成骨分化的骨结节形成的显着增加。通过免疫组织化学，羊水组织显示c-Kit（簇酪氨酸蛋白激酶）、CD 105和Oct-4（八聚体结合转录因子4）抗原呈阳性，证实了具有胚胎表型的MSC的存在。
The amnion is a particular tissue whose cells show features of multipotent stem cells proposed for use in cellular therapy and regenerative medicine. From equine amnion collected after the foal birth we have isolated MSCs (mesenchymal stem cells), namely EAMSCs (equine amnion mesenchymal stem cells), from the mesoblastic layer. The cells were grown in α-MEM (α-modified minimum essential medium) and the effect of EGF (epidermal growth factor) supplementation was evaluated. To assess the growth kinetic of EAMSCs we have taken into account some parameters [PD (population doubling), fold increase and DT (doubling time)]. The differentiation in chondrogenic, adipogenic and osteogenic types of cells and their epitope expression by a cytofluorimetric study have been reported. EGF supplementation of the culture medium resulted in a significant increase in PD growth parameter and in the formation of bone nodules for the osteogenic differentiation. By immunohistochemistry the amnion tissue shows a positivity for the c-Kit (cluster tyrosine-protein kinase), CD105 and Oct-4 (octamer-binding transcription factor 4) antigens that confirmed the presence of MSCs with embryonic phenotype.