Differential transcript profiling alters regulatory gene expression during the development of Gossypium arboreum, G.stocksii and somatic hybrids.
Differential transcript profiling alters regulatory gene expression during the development of Gossypium arboreum, G.stocksii and somatic hybrids.
复制标题
差异转录谱分析改变了树棉、G.stocksii 和体细胞杂种发育过程中的调控基因表达
DOI：
10.1038/s41598-017-03431-3
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发表时间：
2017-06-09
影响因子：
4.6
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Ke L;Luo B;Zhang L;Zhang M;Yu X;Sun J;Sun Y
多倍性或基因组加倍（即，在生物体内存在两个或多个二倍体亲本基因组组）在高等植物中非常重要。特别令人感兴趣的是共同核的新微环境中的机制，其中双重调控网络相互作用，产生一个能够调控生长、发育和对环境反应的可行遗传系统。为了确定全基因组融合和加倍对亚洲棉A1 A1基因组和施氏棉E1 E1基因组原生质体融合产生的一种新多倍体的整体基因表达结构的影响，我们利用cDNA-AFLP技术对体细胞杂种（G. arboreum + G. stocksii）。体细胞杂种的基因组表达模式显示，表达水平的变化主要涉及调控基因（31.8%的基因表达谱），AA和EE基因组的贡献相等，在新形成的AAEE基因组的加性和显性效应的全基因组表达。这些结果提供了一个新的视角多倍体基因调控和暗示异源多倍体适应新的微环境核的潜在遗传基础。
Polyploidy or genome doubling (i.e., the presence of two or more diploid parental genome sets within an organism) are very important in higher plants. Of particular interest are the mechanisms in the new microenvironment of the common nucleus, where doubled regulatory networks interact to generate a viable genetic system capable of regulating growth, development and responses to the environment. To determine the effects of whole genome merging and doubling on the global gene expression architecture of a new polyploid, derived from protoplast fusion of the A1A1 genome of Gossypium arboreum and the E1E1 genome of Gossypium stocksii, we monitored gene expression through cDNA-AFLP in the somatic hybrids (G. arboreum + G. stocksii). The genomic expression patterns of the somatic hybrids revealed that changes in expression levels mainly involved regulatory genes (31.8% of the gene expression profiles), and the AA and EE genomes contributed equally to genome-wide expression in the newly formed AAEE genome from additivity and dominance effects. These results provide a novel perspective on polyploid gene regulation and hint at the underlying genetic basis of allopolyploid adaption in the new microenvironmental nucleus.