Genetic characterization of atypical porcine pestivirus (APPV) in China and the successful isolation of a novel APPV strain within genotype 2
Genetic characterization of atypical porcine pestivirus (APPV) in China and the successful isolation of a novel APPV strain within genotype 2
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DOI：
10.1016/j.micpath.2021.105282
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发表时间：
2021-11-09
影响因子：
3.8
通讯作者：
Cao, Zhi
中科院分区：
文献类型：
--
作者：
Zhang, Qiaoya;Yu, Ying;Cao, Zhi
非典型猪瘟病毒(APPV)是引起A-II型先天性震颤的主要病原之一。本研究建立了中国地区APPV毒株的地理分布和遗传特征。到目前为止，中国至少有十个省/地区检测到了APPV。遗传变异分析表明，NS3基因在中国APPV毒株中高度保守，而NS5A、Npro和Erns编码基因高度变异。系统发育分析表明，所有参比菌株可分为3个基因型(1-3)和7个亚型(1.1-1.7)。中国APPV株均为3种亚型和5种亚型(1.2和1.4-1.7)。非结构蛋白NS3、NS5A和NS5B的氨基酸插入/缺失仅在中国APPV株中存在。在3种囊膜糖基化蛋白中，Erns是糖基化程度最高的蛋白，不同APPV株的E2糖基化位点与基因类型有明显的相关性。此外，在河南省还发现了一株新的APPV毒株中国/HeN01/2018，属于2型。然后将其分离并在猪胚胎肾上皮细胞(SPEV细胞)中成功增殖。这项研究为更好地了解中国地区APPV毒株的分化提供了最新的信息。首次成功分离到一株中国APPV毒株，为今后的研究奠定了基础。
Atypical porcine pestivirus (APPV) is one major causative agent of congenital tremor (CT) type A-II. In this study, the geographical distribution and genetic characteristics of APPV strains in China were established. To date, APPV has been detected in at least ten provinces/regions of China. Genetic variability analysis showed that NS3 genes were highly conserved among Chinese APPV strains, while NS5A-, Npro-, and Erns-encoding genes were highly variable. Phylogenetic analysis revealed that all of the reference strains could be classified into 3 genotypes (1-3) and 7 subgenotypes (1.1-1.7). The Chinese APPV strains were assigned to all 3 genotypes and to 5 subgenotypes (1.2 and 1.4-1.7) of genotype 1. Amino acid insertions/deletions in nonstructural proteins, including NS3, NS5A, and NS5B, could only be found in Chinese APPV strains. Among the three envelope glycosylation proteins (Erns, E1, and E2), Erns was the most heavily glycosylated protein, and the N-glycosylation sites of E2 in different APPV strains showed apparent correlations with genetic types. Furthermore, a novel APPV strain named China/HeN01/2018 belonging to genotype 2 was identified in Henan Province. It was then isolated and successfully propagated in embryonic porcine kidney epithelial cells (SPEV cells). This study provides updated information to better understand the divergence of APPV strains in China. This first successful isolation of a Chinese APPV strain provides a research foundation for future studies.