Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice
Beneficial Effects of Potentilla discolor Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice
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DOI：
10.3390/nu11030670
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发表时间：
2019-03-20
期刊：
影响因子：
5.9
通讯作者：
Mao, Xueying
中科院分区：
文献类型：
--
作者：
Han, Lihua;Li, Tiange;Mao, Xueying
变色委陵菜是一种多年生草本植物，在多种疾病的治疗中得到广泛应用。本研究旨在观察PDB水提物对高脂饮食(HFD)和链脲佐菌素(STZ)诱导的2型糖尿病(T2D)小鼠全身炎症反应和肠道微生物区系的影响。将C57BL/6J小鼠随机分为正常饮食(ND)组、T2D组和PDB组(给予PDB水提物400 mg/kg体重治疗)。结果显示，PDB能显著降低大鼠血清内毒素和促炎细胞因子水平。进一步的研究表明，PDB能显著降低糖尿病小鼠粪便中细菌的比例和变形杆菌的相对丰度。此外，PDB显著减轻肠道炎症，表现为Toll样受体4(TLR4)、髓系分化因子88(MyD88)、核因子-kappaB(NF-kappa B)和炎性细胞因子的表达减少。PDB还逆转了肠粘膜紧密连接蛋白Claudin3、ZO-1和occludin的表达下降。同时，大便中的乙酸、丁酸及其特异性受体G蛋白偶联受体(GPR)41和43的表达水平也明显升高。我们的结果表明，PDB可能是一种潜在的抗糖尿病和相关炎症的功能成分。
Potentilla discolor Bunge (PDB), a perennial herb, has been used as a traditional Chinese medicine in the therapy of many diseases. The aim of the current study was to investigate the effect of PDB water extract on systemic inflammation and gut microbiota in type 2 diabetic (T2D) mice induced by high-fat diet (HFD) and streptozotocin (STZ) injection. C57BL/6J mice were randomly divided into a normal diet (ND) group, T2D group, and PDB group (diabetic mice treated with PDB water extract at a dose of 400 mg/kg body weight). Results showed that PDB significantly decreased the levels of lipopolysaccharide (LPS) and pro-inflammatory cytokines in serum. Further investigation showed that PDB significantly reduced the ratio of Firmicutes/Bacteroidetes and the relative abundance of Proteobacteria in fecal samples of diabetic mice. In addition, PDB notably alleviated intestinal inflammation as evidenced by decreased expression of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor-kappa B (NF-kappa B), and inflammatory cytokines. PDB also reversed the decreased expression of intestinal mucosal tight junction proteins including Claudin3, ZO-1, and Occludin. Meanwhile, the levels of fecal acetic acid and butyric acid and their specific receptors including G-protein-coupled receptor (GPR) 41 and 43 expression in the colon were also increased after PDB treatment. Our results indicated that PDB might serve as a potential functional ingredient against diabetes and related inflammation.