Hemolymph ion composition and volume changes in the supralittoral isopod Ligia pallasii Brandt, during molt
Hemolymph ion composition and volume changes in the supralittoral isopod Ligia pallasii Brandt, during molt
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滨海等足动物 Ligia pallasii Brandt 在蜕皮过程中的血淋巴离子组成和体积变化
DOI：
10.1007/s003600000108
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
S. Pennings
中科院分区：
文献类型：
--
作者：
A. Ziegler;T. Grospietsch;T. Carefoot;J. Danko;M. Zimmer;I. Zerbst;S. Pennings
用毛细管电泳法和~3H-菊粉分析了不同蜕皮周期的巴氏木虱血淋巴的离子组成和体积。血淋巴中的主要离子为Na+、K+、Mg~(2+)、Ca~(2+)和Cl~(−)。蜕皮过程中钙离子浓度从蜕皮到蜕皮内增加了47%，从蜕皮到蜕皮后增加了37%，这可能是由于角质层和胸骨CaCO3沉淀物中的钙离子被吸收所致。蜕皮过程中K+浓度显著增加20%。动物的血淋巴体积以干质量为标准，从中间蜕皮到后期预蜕皮下降了36%。这是由于动物在蜕皮前的血淋巴体积减少和干质量增加所致。在蜕皮后期和蜕皮内，血淋巴体积突然增加，有助于角质层的扩张。由于Na+、K+、Mg~(2+)和Cl~(2+)的−浓度从蜕皮后期到蜕皮内没有显著变化，血淋巴体积的增加表明摄取的是海水而不是淡水。
We analyzed ion composition and volume of the hemolymph ofLigia pallasiiin four different stages of the molt cycle using capillary electrophoresis and3H-inulin. The main ions in the hemolymph were Na+, K+, Mg2+, Ca2+, and Cl−. The Ca2+concentration increased significantly during the molt by 47% from intermolt to intramolt and by 37% from intermolt to postmolt, probably due to resorption of Ca2+from the cuticle and sternal CaCO3deposits. The K+concentration increased significantly by 20% during molt. The hemolymph volume normalized to the dry mass of the animals decreased by 36% from intermolt to late premolt. This was due to a reduction in the hemolymph volume and to an increase in dry mass of the animals during premolt. A sudden increase in the hemolymph volume occurring between late premolt and intramolt served to expand the cuticle. Since the Na+, K+, Mg2+, and Cl−concentrations did not change significantly from late premolt to intramolt, the increase in hemolymph volume suggests an uptake of seawater rather than freshwater.