The enhancement of osteogenesis by nano-fibrous scaffolds incorporating rhBMP-7 nanospheres
The enhancement of osteogenesis by nano-fibrous scaffolds incorporating rhBMP-7 nanospheres
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DOI：
10.1016/j.biomaterials.2006.12.028
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发表时间：
2007-04-01
期刊：
影响因子：
14
通讯作者：
Ma, Peter X.
中科院分区：
文献类型：
--
作者：
Wei, Guobao;Jin, Qiming;Ma, Peter X.
将受控生长因子递送纳入组织工程策略是有利的。本研究的目的是开发一种三维（313）多孔组织工程支架，具有控释重组人骨形态发生蛋白7（rhBMP-7）的能力，以促进骨再生。 RhBMP-7 首先被封装到平均直径为 300 nm 的聚乳酸-乙醇酸 (PLGA) 纳米球 (NS) 中。使用组合的糖球模板浸出和相分离技术制备了具有互连的大孔和纳米纤维结构的聚（L-乳酸）（PLLA）支架。然后利用播种后技术将含有 PLGA 纳米球的 rhBMP-7 固定到具有维护良好的 3D 结构的预制纳米纤维 PLLA 支架上。体外释放动力学表明，纳米球固定支架（NS-支架）可以以时间受控的方式释放rhBMP-7，具体取决于固定在支架上的NS的化学和降解特性。在体内，从 NS 支架递送的 rhBMP-7 在整个支架中诱导显着的异位骨形成，而 rhBMP-7 被动吸附到支架中，由于 rhBMP-7 生物学功能的丧失或在支架内持续时间不足而导致骨诱导失败。我们得出的结论是，互连的大孔结构和从 NS 固定的纳米纤维支架持续、长时间地递送生物活性 rhBMP-7 积极诱导了整个支架的新骨形成。该方法提供了一种新的 BMP 递送方法和用于骨再生的新型支架设计。 (c) 2007 Elsevier Ltd. 保留所有权利。
It is advantageous to incorporate controlled growth factor delivery into tissue engineering strategies. The objective of this study was to develop a three-dimensional (313) porous tissue engineering scaffold with the capability of controlled releasing recombinant human bone morphogenctic protein-7 (rhBMP-7) for enhariccment of bone regeneration. RhBMP-7 was first encapsulated into poly(lactic-co-glycolic acid) (PLGA) nanospheres (NS) with an average diameter of 300nm. Poly(L -lactic acid) (PLLA) scaffolds with interconnected macroporous and nano-fibrous architectures were prepared using a combined sugar sphere template leaching and phase separation technique. A post-seeding technique was then utilized to immobilize rhBMP-7 containing PLGA nanospheres onto prefabricated nanofibrous PLLA scaffolds with well-maintained 3D, structures. In vitro release kinetics indicated that nanosphere immobilized scaffold (NS-scaffold) could release rhBMP-7 in a temporally controlled manner, depending on the chemical and degradation properties of the NS which were immobilized onto the scaffold. In vivo, rhBMP-7 delivered from NS-scaffolds induced significant ectopic bone formation throughout the scaffold while passive adsorption of rhBMP-7 into the scaffold resulted in failure of bone induction due to either the loss of rhBMP-7 biological function or insufficient duration within the scaffold. We conclude that the interconnected macroporous architecture and the sustained, prolonged delivery of bioactive rhBMP-7 from NS immobilized nano-fibrous scaffolds actively induced new bone formation throughout the scaffold. The approach offers a new delivery method of BMPs and a novel scaffold design for bone regeneration. (c) 2007 Elsevier Ltd. All rights reserved.