Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)
Microfocus X-ray CT (microCT) Imaging of Actinia equina (Cnidaria), Harmothoe sp. (Annelida), and Xenoturbella japonica (Xenacoelomorpha)
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DOI：
10.3791/59161
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发表时间：
2019-08-01
影响因子：
1.2
通讯作者：
Nakano, Hiroaki
中科院分区：
文献类型：
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作者：
Maeno, Akiteru;Kohtsuka, Hisanori;Nakano, Hiroaki
传统上，生物学家不得不依靠破坏性的方法，如切片，以研究不透明生物体的内部结构。由于样本染色方法的技术进步以及microCT硬件、处理计算机和数据分析软件的创新，非破坏性微焦点X射线计算机断层扫描（microCT）成像已成为生物学中强大且新兴的协议。然而，该协议并不常用，因为它是在医疗和工业领域。这种有限使用的原因之一是缺乏一个简单易懂的手册，涵盖所有必要的步骤：样品收集，固定，染色，封片，扫描和数据分析。另一个原因是后生动物的多样性，特别是海洋无脊椎动物。由于海洋无脊椎动物的大小，形态和生理的多样性，这是至关重要的，以调整实验条件和硬件配置在每一步，根据样品。在这里，microCT成像方法详细解释使用三种遗传多样的海洋无脊椎动物：海葵（珊瑚虫，刺胞动物），Harmothoe属。（多毛类，环节动物），和Xenoturbella japonica（Xenoturbellida，Xenacoelomorpha）。还提供了对各种动物进行microCT成像的建议。
Traditionally, biologists have had to rely on destructive methods such as sectioning in order to investigate the internal structures of opaque organisms. Non-destructive microfocus X-ray computed tomography (microCT) imaging has become a powerful and emerging protocol in biology, due to technological advancements in sample staining methods and innovations in microCT hardware, processing computers, and data analysis software. However, this protocol is not commonly used, as it is in the medical and industrial fields. One of the reasons for this limited use is the lack of a simple and comprehensible manual that covers all of the necessary steps: sample collection, fixation, staining, mounting, scanning, and data analyses. Another reason is the vast diversity of metazoans, particularly marine invertebrates. Because of marine invertebrates' diverse sizes, morphologies, and physiologies, it is crucial to adjust experimental conditions and hardware configurations at each step, depending on the sample. Here, microCT imaging methods are explained in detail using three phylogenetically diverse marine invertebrates: Actinia equina (Anthozoa, Cnidaria), Harmothoe sp. (Polychaeta, Annelida), and Xenoturbella japonica (Xenoturbellida, Xenacoelomorpha). Suggestions on performing microCT imaging on various animals are also provided.