Carvacrol and 1,8-cineole alone or in combination at sublethal concentrations induce changes in the cell morphology and membrane permeability of Pseudomonas fluorescens in a vegetable-based broth
Carvacrol and 1,8-cineole alone or in combination at sublethal concentrations induce changes in the cell morphology and membrane permeability of Pseudomonas fluorescens in a vegetable-based broth
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DOI：
10.1016/j.ijfoodmicro.2012.06.008
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发表时间：
2012-08-01
影响因子：
5.4
通讯作者：
de Souza, Evandro Leite
中科院分区：
文献类型：
--
作者：
de Sousa, Jossana Pereira;Torres, Rayanne de Araujo;de Souza, Evandro Leite
本研究旨在研究亚致死浓度的香芹酚（CAR）和1，8-桉叶素（ClN）单独和组合对荧光假单胞菌ATCC 11253的形态，细胞活力和膜通透性的影响，在蔬菜为基础的肉汤培养。暴露于CAR和ClN单独或组合的细菌细胞的透射和扫描电子显微镜图像在暴露1小时后显示出显著的超微结构变化。这些变化包括原生质萎缩，外膜和细胞质膜的不连续性和细胞内物质的泄漏。共聚焦扫描激光显微镜图像证实了电子显微镜数据，显示SYTO-9细胞（完整细胞）数量减少，同时PI阳性细胞（死细胞）数量增加。所有这些形态学变化都表明膜渗透性增加和细菌包膜完整性丧失，最终导致细胞死亡。亚致死浓度CAR和ClN的组合可用于抑制蔬菜上荧光假单胞菌的生长。(C)2012 Elsevier B. V.保留所有权利。
This study aimed to investigate the effects of sublethal concentrations of carvacrol (CAR) and 1,8-cineole (ClN) alone and in combination on the morphology, cell viability and membrane permeability of Pseudomonas fluorescens ATCC 11253 cultivated in a vegetable-based broth. Transmission and scanning electron microscopy images of bacterial cells exposed to CAR and ClN alone or in combination showed marked ultrastructural changes after 1 h of exposure. These changes included shrunken protoplasm, discontinuity of the outer and cytoplasmic membranes and leakage of the intracellular material. Confocal scanning laser microscopy images corroborated the electron microscopy data, showing a decrease in the number of SYTO-9 cells (intact cells) with a concomitant increase in the number of PI-positive cells (dead cells). All of these morphological changes are indicative of increased membrane permeability and the loss of bacterial envelope integrity, which ultimately lead to cell death. The combination of sublethal concentrations of CAR and ClN could be applied to inhibit the growth of P. fluorescens on vegetables. (C) 2012 Elsevier B.V. All rights reserved.