Detection of cathepsin S cysteine protease in human brain tumour microdialysates in vivo
Detection of cathepsin S cysteine protease in human brain tumour microdialysates in vivo
复制标题
DOI：
10.1080/02688690701248190
复制
发表时间：
2007-01-01
影响因子：
1.1
通讯作者：
Johnston, P. G.
中科院分区：
文献类型：
--
作者：
Flannery, T.;Mcconnell, R. S.;Johnston, P. G.
微透析使身体组织中细胞外液的化学成分能够被测量。细胞外蛋白酶如半胱氨酸蛋白酶、组织蛋白酶S（CatS）被认为促进星形细胞瘤侵袭。对从体内人脑肿瘤中获得的微透析液进行组织蛋白酶S活性和ELISA测定。在10个肿瘤微透析液中的5个中检测到组织蛋白酶S ELISA表达，而在11个肿瘤微透析液中的5个中检测到活性。组织蛋白酶S的表达也检测到微透析液从正常的大脑控制，虽然没有发现在同一样品中的活动。虽然对该技术进行一些改进是必要的，但作者证明了微透析在体内人类星形细胞瘤中的可行性和安全性。使用微透析表征脑肿瘤在体内的细胞外环境可能是一个有用的工具，以确定脑肿瘤的蛋白酶谱。
Microdialysis enables the chemistry of extracellular fluid in body tissues to be measured. Extracellular proteases such as the cysteine protease, cathepsin S (CatS), are thought to facilitate astrocytoma invasion. Microdialysates obtained from human brain tumours in vivo were subjected to cathepsin S activity and ELISA assays. Cathepsin S ELISA expression was detected in five out of 10 tumour microdialysates, while activity was detected in five out of 11 tumour microdialysates. Cathepsin S expression was also detected in microdialysate from the normal brain control although no activity was found in the same sample. While some refinements to the technique are necessary, the authors demonstrate the feasibility and safety of microdialysis in human astrocytomas in vivo. Characterisation of the extracellular environment of brain tumours in vivo using microdialysis may be a useful tool to identify the protease profile of brain tumours.