Isolation, Characterization, and Phylogenetic Analysis of Two New Crimean-Congo Hemorrhagic Fever Virus Strains from the Northern Region of Xinjiang Province, China
Isolation, Characterization, and Phylogenetic Analysis of Two New Crimean-Congo Hemorrhagic Fever Virus Strains from the Northern Region of Xinjiang Province, China
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DOI：
10.1007/s12250-018-0020-7
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发表时间：
2018-02-01
影响因子：
5.5
通讯作者：
Deng, Fei
中科院分区：
文献类型：
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作者：
Zhang, Yanfang;Shen, Shu;Deng, Fei
克里米亚-刚果出血热 (CCHF) 由克里米亚-刚果出血热 (CCHF) 病毒 (CCHFV) 引起，是一种蜱传自然疫源性疾病，死亡率约为 50%。 CCHFV 在非洲、南亚、中东和东南欧广泛流行。中国新疆省，特别是南部地区此前曾报道过 CCHF 疫情。对蜱虫和动物的流行病学调查显示，南疆各市县的蜱虫、啮齿动物和感染者体内均存在CCHFV毒株。系统发育分析表明，根据其负链RNA基因组S片段的完整序列，中国CCHFV毒株属于一种基因型。本研究报道了从新疆北部地区阜康市和五家渠市采集的亚洲透明蜱蜱中分离出的两株CCHFV新毒株。通过宏基因组和逆转录PCR分析来分析病毒特征及其进化关系；这些分析表明，这两个菌株的基因型与其他中国菌株的基因型不同。此外，还回顾了新疆 CCHFV 的以往报道并进行了系统发育分析。准噶尔盆地阜康市发现CCHFV流行20余年，阜康市和五家渠市被认为是不同基因型CCHFV毒株的天然宿主。我们的研究结果有助于了解新疆省 CCHFV 的分布，并为中国 CCHFV 毒株之间的进化关系提供见解。
Crimean-Congo hemorrhagic fever (CCHF) caused by the CCHF virus (CCHFV) is a tick-borne natural focal disease with a mortality rate of approximately 50%. CCHFV is widely prevalent in Africa, southern Asia, the Middle East, and southeast Europe. CCHF outbreaks have been reported previously in Xinjiang province, China, especially in its southern region. Epidemiological surveys conducted on ticks and animals have revealed the presence of CCHFV strains in ticks, rodents, and infected individuals from cities and counties in southern Xinjiang. Phylogenetic analyses revealed that the Chinese CCHFV strains belong to one genotype, based on complete sequences of the S segments of its negative-stranded RNA genome. The present study reports two new CCHFV strains isolated from Hyalomma asiaticum asiaticum ticks collected from Fukang City and Wujiaqu City in the northern region of Xinjiang. Viral characteristics and their evolutionary relationships were analyzed through metagenomic and reverse-transcription PCR analyses; these analyses indicated that the genotype of both strains was different from that of other Chinese strains. Furthermore, previous reports of CCHFV in Xinjiang were reviewed and phylogenetic analyses were performed. CCHFV was found to prevail in Fukang City in Junggar Basin for more than 20 years, and that Fukang City and Wujiaqu City are considered natural reservoirs of different genotypes of CCHFV strains. Our findings facilitate the understanding of CCHFV distribution in Xinjiang province and provide insights into the evolutionary relationships among Chinese CCHFV strains.