EXPRESSION OF OUTER SURFACE-PROTEIN-A AND SURFACE-PROTEIN-C OF BORRELIA-BURGDORFERI IN IXODES-RICINUS
EXPRESSION OF OUTER SURFACE-PROTEIN-A AND SURFACE-PROTEIN-C OF BORRELIA-BURGDORFERI IN IXODES-RICINUS
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DOI：
10.1128/jcm.33.7.1867-1869.1995
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发表时间：
1995-07-01
影响因子：
9.4
通讯作者：
WILSKE, B
中科院分区：
文献类型：
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作者：
FINGERLE, V;HAUSER, U;WILSKE, B
采用多价兔免疫血清和外表面蛋白A和C（分别为OspA和OspC）特异性单克隆抗体，通过免疫荧光法研究了来自德国南部的472只野外采集的蓖麻硬蜱中是否存在伯氏疏螺旋体。用多价免疫荧光法对90只蜱进行了包柔螺旋体检测。成年人的感染率（女性，20.2%;男性，25.2%）明显高于成年人（12.1%）。用相应的单克隆抗体在77只蜱中检测到OspA，仅在1只蜱中检测到OspC。因此，我们得出结论，B。布格多费里因恩菲德岛蓖麻蜱通常表达OspA，很少表达OspC。
A total of 472 field-collected Ixodes ricinus ticks from southern Germany were investigated by immunofluorescence for the presence of Borrelia burgdorferi with a polyvalent rabbit immune serum and,vith monoclonal antibodies specific for outer surface proteins A and C (OspA and OspC, respectively). Borreliae were detected in 90 ticks with the polyvalent immunofluorescence assay. Infection rates in adults (females, 20.2%; males, 25.2%) were significantly higher than in nymphs (12.1%). OspA was detected in 77 ticks and OspC was detected in only 1 tick with the respective monoclonal antibodies. We therefore conclude that B. burgdorferi in unfed I. ricinus ticks usually expresses OspA and very rarely OspC.