Neutrophils play an essential role in cooperation with antibody in both protection against and recovery from pulmonary infection with influenza virus in mice
Neutrophils play an essential role in cooperation with antibody in both protection against and recovery from pulmonary infection with influenza virus in mice
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DOI：
10.1128/jvi.01210-07
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发表时间：
2008-03-01
影响因子：
5.4
通讯作者：
Fujisawa, Haruo
中科院分区：
文献类型：
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作者：
Fujisawa, Haruo
通过去除肺部感染小鼠中的 PMN 以及被动转移抗流感病毒抗血清，研究了多形核白细胞 (PMN) 在甲型流感病毒感染早期的保护和晚期恢复中的作用。用单克隆抗体RB6-8C5治疗正常小鼠中的PMN，从感染后早期到晚期均增加了死亡率和肺部病毒滴度，并延迟了晚期病毒消除。在感染前或感染后将抗血清被动转移至正常小鼠体内，可分别在感染后的早期阶段（3天）消除肺部病毒的繁殖，或在平台期（第3至5天）迅速降低高病毒滴度，并在感染后的后期（第7天）加速病毒消除。与对照组相比，将抗血清被动转移到PMN耗尽的小鼠体内既不能阻止早期阶段更快的病毒传播，也不能降低平台期较高的病毒滴度，也不能加速感染后后期明显延迟的病毒消除。正常小鼠和 PMN 耗尽的小鼠在感染后第 7 天，对病毒的抗体反应开始增强。在抗血清的存在下，病毒复制的预防、病毒感染的细胞培养物中的细胞毒活性以及PMN对病毒的体外吞噬作用均得到增强。这些结果表明，PMN 与抗体反应相配合，在消除病毒、预防感染和恢复感染方面发挥着重要作用。
The role of polymorphonuclear leukocytes (PMN) in protection in the early phase and recovery in the late phase of influenza A virus infection was investigated by the depletion of PMN in, and passive transfer of anti-influenza virus antiserum to, mice with pulmonary infections. The depletion of PMN in normal mice by treatment with monoclonal antibody RB6-8C5 both increased the mortality rate and pulmonary virus titers from the early to the late phase after infection and delayed virus elimination in the late phase. The passive transfer of the antiserum to normal mice before or after infection abolished pulmonary virus propagation in the early phase, during 3 days, or rapidly decreased high virus titers in the plateau phase, on days 3 to 5, as well as accelerated virus elimination in the late phase, on day 7, after infection, respectively. The passive transfer of the antiserum to PMN-depleted mice could neither prevent the more rapid virus propagation in the early phase, diminish the higher virus titers in the plateau phase, nor accelerate the markedly delayed virus elimination in the late phase after infection in comparison to those for controls. The antibody responses to the virus began to increase on day 7 after infection in normal and PMN-depleted mice. The prevention of virus replication, cytotoxic activity in virus-infected cell cultures, and phagocytosis of the virus in vitro by PMN were all augmented in the presence of the antiserum. These results indicate that PMN play an essential role in virus elimination in both protection against and recovery from infection, in cooperation with the antibody response.