Imaging dedicated and multifunctional neural circuits generating distinct behaviors
Imaging dedicated and multifunctional neural circuits generating distinct behaviors
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DOI：
10.1523/jneurosci.3265-06.2006
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发表时间：
2006-10-18
影响因子：
5.3
通讯作者：
Kristan, William B., Jr.
中科院分区：
文献类型：
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作者：
Briggman, Kevin L.;Kristan, William B., Jr.
中央模式发生器(CPGs)控制着药用水蚤的游动和爬行。为了研究组成这两个CPG的神经元是专用的还是多功能的，我们使用电压敏感染料成像技术记录了节段性神经节中类似于400个神经元中的类似神经元的80%。通过在相同的准备中激发游泳和爬行，我们能够识别参与这两种节奏中的一种或两种的神经元。爬行(188个)与游泳(90个)同步振荡的细胞数量是游泳组(90个)的两倍多。令人惊讶的是，84个(93%)随着游泳而振荡的细胞也随着爬行而振荡。然后，我们根据成像结果描述了两个先前未被识别的中间神经元，细胞255和257，具有有趣的活动模式。细胞255被证明是一个多功能的中间神经元，可以随着这两种节律振荡并可以扰乱它们，而细胞257是一个致力于爬行的中间神经元。这些结果表明，游泳和爬行网络是由多功能电路和专用电路驱动的。
Central pattern generators (CPGs) control both swimming and crawling in the medicinal leech. To investigate whether the neurons comprising these two CPGs are dedicated or multifunctional, we used voltage- sensitive dye imaging to record from similar to 80% of the similar to 400 neurons in a segmental ganglion. By eliciting swimming and crawling in the same preparation, we were able to identify neurons that participated in either of the two rhythms, or both. More than twice as many cells oscillated in- phase with crawling (188) compared with swimming (90). Surprisingly, 84 of the cells (93%) that oscillated with swimming also oscillated with crawling. We then characterized two previously unidentified interneurons, cells 255 and 257, that had interesting activity patterns based on the imaging results. Cell 255 proved to be a multifunctional interneuron that oscillates with and can perturb both rhythms, whereas cell 257 is an interneuron dedicated to crawling. These results show that the swimming and crawling networks are driven by both multifunctional and dedicated circuitry.