An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region.
An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region.
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拟南芥肌球蛋白XI的同工型与其C末端尾部区域的小GTPase相互作用。
DOI：
10.1093/jxb/ern202
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发表时间：
2008
影响因子：
6.9
通讯作者：
Yokota, Etsuo
中科院分区：
文献类型：
--
作者：
Hashimoto, Kohsuke;Igarashi, Hisako;Mano, Shoji;Takenaka, Chikako;Shiina, Takashi;Yamaguchi, Masatoshi;Demura, Taku;Nishimura, Mikio;Shimmen, Teruo;Yokota, Etsuo
肌球蛋白XI是一类在植物中表达的肌球蛋白，被认为与细胞质流动以及细胞器和囊泡的移位有关。为了进一步了解肌球蛋白XI对细胞器和囊泡的转运，我们选择了拟南芥肌球蛋白XI的一个亚型MYA2，并研究了它在过氧化物酶体靶向中的作用。利用酵母双杂交筛选方法，确定了两个小的GTP酶AtRabD1和AtRabC2a，它们是与MYA2的C末端尾区相互作用的因子。His标记的重组AtRabs均以GTP依赖的方式与GST标记的重组MYA2的C末端尾区结合。此外，当AtRabC2a的CFP标记形式在拟南芥叶组织制备的原生质体中瞬时表达时，AtRabC2a定位于过氧化体上。提示MYA2通过与AtRabC2a相互作用来靶向过氧化物酶体。
Myosin XI, a class of myosins expressed in plants is believed to be responsible for cytoplasmic streaming and the translocation of organelles and vesicles. To gain further insight into the translocation of organelles and vesicles by myosin XI, an isoform of Arabidopsis myosin XI, MYA2, was chosen and its role in peroxisome targeting was examined. Using the yeast two-hybrid screening method, two small GTPases, AtRabD1 and AtRabC2a, were identified as factors that interact with the C-terminal tail region of MYA2. Both recombinant AtRabs tagged with His bound to the recombinant C-terminal tail region of MYA2 tagged with GST in a GTP-dependent manner. Furthermore, AtRabC2a was localized on peroxisomes, when its CFP-tagged form was expressed transiently in protoplasts prepared from Arabidopsis leaf tissue. It is suggested that MYA2 targets the peroxisome through an interaction with AtRabC2a.
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