Genome-wide analysis of the GH3 family in apple (Malus × domestica).
Genome-wide analysis of the GH3 family in apple (Malus × domestica).
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DOI：
10.1186/1471-2164-14-297
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发表时间：
2013-05-02
期刊：
影响因子：
4.4
通讯作者：
Li T
中科院分区：
文献类型：
--
作者：
Yuan H;Zhao K;Lei H;Shen X;Liu Y;Liao X;Li T
生长素在激素串扰和植物的胁迫反应中起着重要作用。生长素应答基因Gretchen Hagen3 （GH3）家族通过在激素和应激相关的信号通路中将过量的吲哚-3-乙酸（IAA）、水杨酸（SA）和茉莉酸（JAs）偶联到氨基酸上来维持激素稳态。随着苹果（Malus × domestica）基因组测序的完成，对GH3基因进行基因组研究以鉴定在非生物/生物胁迫反应中起作用的候选基因成为可能。我是罗马人。以苹果栽培品种的祖先种、耐旱性强的苹果砧木为试验材料。通过对MdGH3基因的全基因组计算和实验鉴定，我们发现MdGH3基因在M. sieversii的叶片和根系中存在差异表达，其中一些基因在各种植物激素和非生物胁迫处理后被显著诱导。考虑到GH3对游离IAA浓度的负反馈调节作用，我们研究了植物激素和非生物胁迫是否会改变内源生长素水平。通过分析DR5::GUS转化的拟南芥幼苗GUS活性，我们发现ABA、SA、盐和冷处理均抑制了生长素的反应。这些发现表明，其他植物激素和非生物应激因素可能改变内源性生长素水平。先前的研究表明，GH3基因调节激素稳态。我们的研究表明，在各种植物激素和非生物胁迫处理下，一些GH3基因在sieversii中被显著诱导，ABA、SA、盐和冷处理降低了axuin的内源水平。综上所述，本研究提供了GH3基因通过维持生长素稳态，在生长素与其他植物激素之间的串扰以及非生物胁迫反应中发挥重要作用的证据。
Auxin plays important roles in hormone crosstalk and the plant’s stress response. The auxin-responsive Gretchen Hagen3 (GH3) gene family maintains hormonal homeostasis by conjugating excess indole-3-acetic acid (IAA), salicylic acid (SA), and jasmonic acids (JAs) to amino acids during hormone- and stress-related signaling pathways. With the sequencing of the apple (Malus × domestica) genome completed, it is possible to carry out genomic studies on GH3 genes to indentify candidates with roles in abiotic/biotic stress responses. Malus sieversii Roem., an apple rootstock with strong drought tolerance and the ancestral species of cultivated apple species, was used as the experimental material. Following genome-wide computational and experimental identification of MdGH3 genes, we showed that MdGH3s were differentially expressed in the leaves and roots of M. sieversii and that some of these genes were significantly induced after various phytohormone and abiotic stress treatments. Given the role of GH3 in the negative feedback regulation of free IAA concentration, we examined whether phytohormones and abiotic stresses could alter the endogenous auxin level. By analyzing the GUS activity of DR5::GUS-transformed Arabidopsis seedlings, we showed that ABA, SA, salt, and cold treatments suppressed the auxin response. These findings suggest that other phytohormones and abiotic stress factors might alter endogenous auxin levels. Previous studies showed that GH3 genes regulate hormonal homeostasis. Our study indicated that some GH3 genes were significantly induced in M. sieversii after various phytohormone and abiotic stress treatments, and that ABA, SA, salt, and cold treatments reduce the endogenous level of axuin. Taken together, this study provides evidence that GH3 genes play important roles in the crosstalk between auxin, other phytohormones, and the abiotic stress response by maintaining auxin homeostasis.