Estrogen Receptor Beta Mediates the Dipsogenic Effect of Estradiol in Ovariectomized Female Rats
Estrogen Receptor Beta Mediates the Dipsogenic Effect of Estradiol in Ovariectomized Female Rats
复制标题
DOI：
10.1159/000530962
复制
发表时间：
2023-05-12
影响因子：
4.1
通讯作者：
Edwards，Andrea A.
中科院分区：
文献类型：
--
作者：
Santollo，Jessica;Edwards，Andrea A.
虽然雌二醇的液体抑制作用的特点，最近确定的激素的致渴作用。在卵巢切除（OVX）大鼠中，在没有食物的情况下，雌二醇治疗后增加了未刺激的水摄入量，这些实验的目的是通过确定介导致渴效应的雌激素受体亚型，检查盐水摄入量，结果雌激素受体β（ERβ）的药理学激活增加了水的摄入量，在没有食物的情况下，并与摄食后反馈信号的变化有关。令人惊讶的是，即使在没有食物的情况下，ERα的激活也会减少水的摄入量。一项随访研究表明，当有食物时，ERα和ERβ的共激活减少了水的摄入量，但当没有食物时，水的摄入量增加。此外，在OVX大鼠中，雌二醇通过改变摄食后和口腔感觉反馈信号增加盐水摄入量。最后，虽然雌二醇减少水的摄入量在雄性大鼠获得食物，雌二醇没有影响水的摄入量在foods.ConclusionsThese结果表明，dipsogenic效应是由ERβ介导的，雌二醇的液体增强作用泛化到盐水，并仅限于女性，这意味着女性化的大脑是必要的雌二醇增加水的摄入量。这些发现将有助于指导未来的研究，重点是阐明允许雌二醇增加和减少液体摄入的神经机制。
IntroductionAlthough the fluid inhibitory effects of estradiol are well characterized, a dipsogenic role of the hormone was recently identified. In ovariectomized (OVX) rats, unstimulated water intake, in the absence of food, was increased after estradiol treatment.MethodsThe goals for these experiments were to further characterize the fluid enhancing effects of estradiol by determining the estrogen receptor subtype mediating the dipsogenic effect, examining saline intake, and testing for a dipsogenic effect of estradiol in male rats.ResultsPharmacological activation of estrogen receptor beta (ERβ) increased water intake, in the absence of food, and was associated with changes in postingestive feedback signals. Surprisingly, activation of ERα reduced water intake even in the absence of food. A follow-up study demonstrated that when food was available, co-activation of ERα and ERβ reduced water intake, but when food was not available water intake was increased. In addition, in OVX rats, estradiol increased saline intake through changes in postingestive and orosensory feedback signals. Finally, although estradiol decreased water intake in male rats with access to food, estradiol had no effect on water intake in the absence of food.ConclusionsThese results demonstrate that the dipsogenic effect is mediated by ERβ, the fluid enhancing effects of estradiol generalize to saline, and is limited to females, which implies that a feminized brain is necessary for estradiol to increase water intake. These findings will aid in guiding future studies focused on elucidating the neuronal mechanisms that allow estradiol to both increase and decrease fluid intake.