Comparative decay of sewage-associated marker genes in beach water and sediment in a subtropical region
Comparative decay of sewage-associated marker genes in beach water and sediment in a subtropical region
复制标题
DOI：
10.1016/j.watres.2018.10.088
复制
发表时间：
2019-02-01
期刊：
影响因子：
12.8
通讯作者：
Simpson, Stuart
中科院分区：
文献类型：
--
作者：
Ahmed, Warish;Zhang, Qian;Simpson, Stuart
越来越多的人倾向于使用基于定量聚合酶链（qPCR）的污水相关标记基因来评估地表水质量。然而，对许多与污水相关的标志物的持久性缺乏了解，给那些负责调查微生物水质的人带来了不确定性。在这项研究中，我们研究了两个qPCR FIB的衰减[E。大肠杆菌（EC）、肠球菌（ENT） 23S rRNA基因]和四种污水相关微生物源追踪（MST）标记基因[人类拟杆菌HF183 16S rRNA、腺病毒（hav）、多瘤病毒（HPyV）和粪便中最近发现的噬菌体crAssphage]，在含有淡水和海水及其相应沉积物的室外中生态系统中。各中观水样中EC 23S rRNA、ENT 23S rRNA和HF183 16S rRNA的衰减速率均显著高于hav、HPyV和crasspge标记物（p < 0.05）。一般来说，细菌目标的腐烂率在研究的中生态系统的水柱中是相似的。同样，病毒靶点的衰减率在中生态水柱中也相似。与三个中生态系统的沉积物相比，水样中FIB和污水相关标志物的衰减速度明显快于沉积物。在再悬浮的情况下，来自沉积物的FIB和标记基因可能会重新污染上覆水域。因此，在解释水中FIB和与污水相关的MST标记物的发生时应小心，它们可能起源于沉积物。这些指标的不同衰减也可能影响健康结果，需要在风险评估模型中加以考虑。爱思唯尔有限公司2018年版权所有版权所有。
There is a growing move towards using the quantitative polymerase chain (qPCR)-based sewage-associated marker genes to assess surface water quality. However, a lack of understanding about the persistence of many sewage-associated markers creates uncertainty for those tasked with investigating microbial water quality. In this study, we investigated the decay of two qPCR FIB [E. coli (EC), and Enterococcus spp. (ENT) 23S rRNA genes] and four sewage-associated microbial source tracking (MST) marker genes [human Bacteroides HF183 16S rRNA, adenovirus (HAdV), and polyomavirus (HPyV), and crAssphage, a recently described bacteriophage in feces], in outdoor mesocosms containing fresh and marine waters and their corresponding sediments. Decay rates of EC 23S rRNA, ENT 23S rRNA, and HF183 16S rRNA were significantly (p < 0.05) faster than the HAdV, HPyV and crAssphage markers in water samples from all mesocosms. In general, decay rates of bacterial targets were similar in the water columns of the studied mesocosms. Similarly, decay rates of viral targets were also alike in mesocosm water columns in relation to each other. The decay rates of FIB and sewage-associated markers were significantly faster in water samples compared to sediments in all three mesocosms. In the event of resuspension, FIB and marker genes from sediments can potentially recontaminate overlying waters. Thus, care should be taken when interpreting the occurrence of FIB and sewage-associated MST markers in water, which may have originated from sediments. The differential decay of these targets may also influence health outcomes and need to be considered in risk assessment models. Crown Copyright (C) 2018 Published by Elsevier Ltd. All rights reserved.