Multiple, heterogeneous actin genes in Dictyostelium
Multiple, heterogeneous actin genes in Dictyostelium
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盘基网柄菌中多个异质肌动蛋白基因
DOI：
10.1016/0092-8674(78)90264-7
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发表时间：
1978
期刊：
影响因子：
64.5
通讯作者：
N. Davidson
中科院分区：
文献类型：
--
作者：
M. Mckeown;W. Taylor;K. Kindle;R. Firtel;W. Bender;N. Davidson
我们使用质粒M6的含肌动蛋白基因的限制性片段（Kindle和Firtel，1976）来选择第二个含肌动蛋白基因的质粒，我们将其命名为pDd肌动蛋白2。该质粒已被证明含有两个肌动蛋白基因，由350 bp的非肌动蛋白DNA分开。当嵌合质粒中存在的三个肌动蛋白基因中的任何两个之间形成异源双链体时，同源区域为1100 ± 100 bp。这接近编码肌动蛋白所需的最小长度。基因间同源性的1100 bp区域对应于在M6和两种肌动蛋白cDNA质粒pcDd肌动蛋白B1和pcDd肌动蛋白A1之间观察到的1100 bp同源性（Bender等人，1976年）。我们没有证据表明在1100和100 bp基因间同源区的3 '或5'端有其他共同序列。热变性实验表明，不同的肌动蛋白基因对彼此偏离多达6- 6%。有两个大小类的mRNA互补的三个肌动蛋白基因。这些具有1.25和1.35 kb的长度，如在含有甲基汞氢氧化物的琼脂糖凝胶上测定的。这三个肌动蛋白基因与其他肌动蛋白基因的可能联系进行了讨论。
We have used an actin gene-containing restriction fragment of plasmid M6 (Kindle and Firtel, 1976) to select a second actin gene-containing plasmid which we have named pDd actin 2. This plasmid has been shown to’contain two actin genes separated by 350 bp of nonactin DNA. When heteroduplexes are formed between any two of the three actin genes present in chimeric plasmids, the region of homology is 1100+ 100 bp. This is close to the minimum length required to code for actin protein. The 1100 bp region of intergene homology corresponds to the 1100 bp homology observed between M6 and the two actin cDNA plasmids pcDd actin Bl and pcDd actin Al (Bender et al., 1976). We have no evidence for additional sequences common to either the 3’or 5’ends of the 1100 & 100 bp region of intergene homology. Thermal denaturation experiments show that different pairs of actin genes are diverged from each other by as much as 6-6%. There are two size classes of mRNA complementary to the three actin genes. These have lengths of 1.25 and 1.35 kb as determined on methyl mercuric hydroxide-containing agarose gels. The possible linkage of these three actin genes to other actin genes is discussed.