ANTIMALARIAL ANTIBODIES OF THE IMMUNOGLOBULIN G2A ISOTYPE MODULATE PARASITEMIAS IN MICE INFECTED WITH PLASMODIUM-YOELII
ANTIMALARIAL ANTIBODIES OF THE IMMUNOGLOBULIN G2A ISOTYPE MODULATE PARASITEMIAS IN MICE INFECTED WITH PLASMODIUM-YOELII
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DOI：
10.1128/iai.59.10.3547-3554.1991
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发表时间：
1991-10-01
影响因子：
3.1
通讯作者：
TAYLOR, DW
中科院分区：
文献类型：
--
作者：
WHITE, WI;EVANS, CB;TAYLOR, DW
以前的研究已经证明了抗体在介导疟疾免疫中的重要性，但不同免疫球蛋白同种型的相对贡献尚未得到评估。 在本研究中，针对约氏疟原虫产生超免疫血浆，并通过蛋白A-琼脂糖色谱法分离成含有免疫球蛋白G1（IgG 1）、IgG 2a、IgG 2b或IgG 3抗体和其余非结合血浆蛋白（包括IgM）的组分。 浓缩后，各同种型组分的抗疟滴度大致相当于超免疫血浆中的相应同种型。 在用毒性约氏疟原虫17 XL攻击之前，将同种型部分被动转移到BALB/c和远系繁殖的ICR小鼠中，并转移到用无毒力约氏疟原虫17 XNL攻击的CBA/CaJ小鼠中。 只有接受IgG 2a抗体的小鼠经历了感染过程的改变。 免疫沉淀研究表明，所有四种IgG同种型似乎识别一组相似的抗原。 这些结果表明，抗疟抗体的IgG 2a同种型发挥主导作用，在调制约氏疟原虫寄生虫血症。
Previous studies have demonstrated the importance of antibodies in mediating immunity to malaria, but the relative contribution of the different immunoglobulin isotypes has not been assessed. In this study, hyperimmune plasma was generated against Plasmodium yoelii and separated by protein A-Sepharose chromatography into fractions containing immunoglobulin G1 (IgG1), IgG2a, IgG2b, or IgG3 antibodies and the remaining nonbinding plasma proteins, including IgM. Following concentration, the antimalarial titer of each isotypic fraction was approximately equivalent to the corresponding isotype in hyperimmune plasma. The isotypic fractions were passively transferred to BALB/c and outbred ICR mice prior to challenge with virulent P. yoelii 17XL and to CBA/CaJ mice challenged with avirulent P. yoelii 17XNL. Only mice receiving IgG2a antibodies experienced an altered course of infection. Immunoprecipitation studies showed that all four IgG isotypes appear to recognize a similar set of antigens. These results suggest that antimalarial antibodies of the IgG2a isotype play a dominant role in modulating P. yoelii parasitemias.