CDNA SEQUENCES OF 3 SHEEP MYELOID CATHELICIDINS
CDNA SEQUENCES OF 3 SHEEP MYELOID CATHELICIDINS
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DOI：
10.1016/0014-5793(95)01285-3
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发表时间：
1995-12-04
期刊：
影响因子：
3.5
通讯作者：
ZANETTI, M
中科院分区：
文献类型：
--
作者：
BAGELLA, L;SCOCCHI, M;ZANETTI, M
几种髓系抗菌肽前体已被证明由一个类似于cathelin蛋白的n端前区和一个结构变化的c端抗菌结构域组成。具有这些特征的蛋白质被命名为cathelicidins。本文报道了三种绵羊抗菌肽的cDNA序列，分别为155、160和190个残基，其阳离子c端序列与推测的抗菌结构域相对应。它们在结构上各不相同，包括一个富含cys的12个残基序列，类似于牛的抗菌环十二肽，一个名为SMAP-29的新型29个残基序列，可能具有α -螺旋构象，以及一个名为Bac7.5的60个残基序列，它似乎是哺乳动物抗菌肽中富含Pro-和arg的新成员。
Several myeloid antimicrobial peptide precursors have been shown to consist of a N-terminal proregion similar to a protein named cathelin and a structurally varied C-terminal antimicrobial domain. Proteins with these features have been named cathelicidins. In this paper me report the cDNA sequences of three ovine cathelicidins of 155, 160 and 190 residues, respectively, with cationic C-terminal sequences corresponding to putative antimicrobial domains. These are structurally varied and include a Cys-rich sequence of 12 residues, which is similar to the bovine antimicrobial cyclic dodecapeptide, a novel 29 residue sequence named SMAP-29 with a possible alpha-helical conformation, and a 60 residue sequence named Bac7.5, which appears to be a new member of the Pro- and Arg-rich group of mammalian antimicrobial peptides.