Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation.
Drosophila Piwi functions in Hsp90-mediated suppression of phenotypic variation.
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DOI：
10.1038/ng.743
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发表时间：
2011-02
期刊：
影响因子：
30.8
通讯作者：
Lin, Haifan
中科院分区：
文献类型：
--
作者：
Gangaraju, Vamsi K.;Yin, Hang;Weiner, Molly M.;Wang, Jianquan;Huang, Xiao A.;Lin, Haifan
管化，也被称为发育稳健性，描述了生物体在基因型变异和环境影响下产生相同表型的能力。在果蝇中，Hsp90、三胸腺组蛋白和转座子沉默先前与管道化有关。尽管如此，渠化的分子机制仍然难以捉摸。本研究使用显性KrIf-1等位基因致敏的aDrosophilaeye-outgrowth assay，我们发现Piwi-interacting RNA （piRNA）通路，而不是短干扰RNA或微RNA通路，参与了管道化。此外，我们分离了一个由Hsp90、Piwi和Hop组成的蛋白复合物，即Hsp70/Hsp90组织蛋白同源物，并证明了该复合物在管道化中的功能。我们的数据表明，Hsp90和Hop通过Piwi调节piRNA通路介导通路。此外，他们指出，现有遗传变异表达的表观遗传沉默和转座子诱导的新遗传变异的抑制是piRNA途径介导的管道化的两个主要机制。
Canalization, also known as developmental robustness, describes an organism's ability to produce the same phenotype despite genotypic variations and environmental influences,. InDrosophila, Hsp90, the trithorax-group proteins and transposon silencing have been previously implicated in canalization,. Despite this, the molecular mechanism underlying canalization remains elusive. Here using aDrosophilaeye-outgrowth assay sensitized by the dominantKrirregular facets-1(KrIf-1) allele, we show that the Piwi-interacting RNA (piRNA) pathway, but not the short interfering RNA or micro RNA pathway, is involved in canalization. Furthermore, we isolated a protein complex composed of Hsp90, Piwi and Hop, the Hsp70/Hsp90 organizing protein homolog, and we demonstrated the function of this complex in canalization. Our data indicate that Hsp90 and Hop regulate the piRNA pathway through Piwi to mediate canalization. Moreover, they point to epigenetic silencing of the expression of existing genetic variants and the suppression of transposon-induced new genetic variation as two major mechanisms underlying piRNA pathway-mediated canalization.
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