Inhibition of testosterone metabolism in cultured rat epididymal principal cells by dihydrotestosterone and progesterone.
Inhibition of testosterone metabolism in cultured rat epididymal principal cells by dihydrotestosterone and progesterone.
复制标题
二氢睾酮和黄体酮对培养的大鼠附睾主细胞睾酮代谢的抑制作用。
DOI：
10.1095/biolreprod30.1.67
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发表时间：
1984
影响因子：
3.6
通讯作者：
Amann,RP
中科院分区：
文献类型：
--
作者：
Brown,DV;Amann,RP
为了评估类固醇通过睾丸输尿管进入附睾对附睾上皮睾酮(T)代谢的影响，通过酶解和洗脱从附睾近端、远端或体分离主细胞，并在34°C的漂浮胶原基质中培养。在培养基中添加近似生理浓度的T、二氢睾酮（DHT）、T +雌二醇-17β(T + E)或T +孕酮（T + P）。DHT孵育2.5 d的主细胞对T的代谢低于对照细胞（P<0.05）。在培养基中加入E或P可降低各区域主细胞对T的代谢（P<0.05）。然而，主细胞经T + P培养2.5 d后，再经T单独洗涤培养12 h，其T代谢与未暴露于P的细胞一样（P<0.05）。与DHT的持续抑制作用明显相反，P对T代谢的抑制作用是快速、直接且快速可逆的。因此，附睾上皮主细胞对T的代谢可能受到睾丸输尿管液中的类固醇（E + P）或附睾局部产生的类固醇（DHT）的调节。
To evaluate the effects of steroids entering the epididymis in rete testis fluid on testosterone (T) metabolism by the epididymal epithelium, principal cells were isolated from the proximal caput, distal caput or corpus epididymidis by enzymatic dissociation and elutriation and were cultured at 34°C within a floating collagen matrix. The culture medium was supplemented with T, dihydrotestosterone (DHT), T plus estradiol-17β(T + E) or T plus progesterone (T + P) at concentrations which were approximately physiologic. Metabolism of T by principal cells incubated for 2.5 days with DHT was lower (P<0.05) than for control cells cultured with T. Inclusion of E or P in the culture medium lowered (P<0.05) metabolism of T by principal cells from each region. However, principal cells cultured with T + P for 2.5 days and then washed and cultured for 12 h with T alone, metabolized T as well (P<0.05) as cells never exposed to P. In marked contrast to the persistent suppressive effect of DHT, the suppressive effect of P on metabolism of T is rapid, direct and rapidly reversible. Thus, metabolism of T by principal cells in the epididymal epithelium may be modulated by steroids (E + P) in rete testis fluid or by steroids (DHT) produced locally in the epididymis.