Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium.
Identification of Stem Leydig Cells Derived from Pig Testicular Interstitium.
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DOI：
10.1155/2017/2740272
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发表时间：
2017
影响因子：
4.3
通讯作者：
Pan C
中科院分区：
文献类型：
--
作者：
Yu S;Zhang P;Dong W;Zeng W;Pan C
干细胞位于哺乳动物睾丸间质中，具有向合成睾酮的间质细胞分化的能力，为治疗睾酮缺乏提供了新的策略。然而，此前还没有报道鉴定和培养猪来源的SLCs。本研究的目的是从猪身上分离、鉴定和培养SLCs。苏木精-伊红染色和免疫组织化学分析表明，猪睾丸中存在SLCs，PDGFRα主要表达于睾丸间质，提示PDGFRα是新生猪SLCs的标志物。此外，逆转录-聚合酶链式反应结果显示，SLC标志物在原代分离的LCs中有表达，表明它们可能是SLC。随后用仔猪睾丸液(PTF)培养液培养可能的SLCs。7d后形成克隆，细胞表达PDGFRα。然而，在没有PTF的情况下，没有克隆生长，但细胞表达了CYP17A1，这表明PTF可以维持猪SLCs的特征。综上所述，我们分离并鉴定了猪SLCs的基本特性。综上所述，这些结果可能有助于为猪SLCs的临床应用研究奠定基础。
Stem Leydig cells (SLCs), located in the testicular interstitial compartment in the mammalian testes, are capable of differentiating to testosterone-synthesizing Leydig cells (LCs), thus providing a new strategy for treating testosterone deficiency. However, no previous reports have identified and cultured SLCs derived from the pig. The aim of the current study was to isolate, identify, and culture SLCs from pigs. Haematoxylin and eosin staining and immunochemical analysis showed that SLCs were present and that PDGFRα was mainly expressed in the pig testicular interstitium, indicating that PDGFRα was a marker for SLCs in the neonatal pig. In addition, reverse transcription-PCR results showed that SLC markers were expressed in primary isolated LCs, indicating that they were putative SLCs. The putative SLCs were subsequently cultured with a testicular fluid of piglets (pTF) medium. Clones formed after 7 days and the cells expressed PDGFRα. However, no clones grew in the absence of pTF, but the cells expressed CYP17A1, indicating that pTF could sustain the features of porcine SLCs. To summarize, we isolated porcine SLCs and identified their basic characteristics. Taken together, these results may help lay the foundation for research in the clinical application of porcine SLCs.