Transcriptional switch from albumin to alpha-fetoprotein and changes in transcription of other genes during carbon tetrachloride induced liver regeneration.
Transcriptional switch from albumin to alpha-fetoprotein and changes in transcription of other genes during carbon tetrachloride induced liver regeneration.
复制标题
在四氯化碳诱导肝再生过程中从白蛋白到甲胎蛋白的转录转换以及其他基因转录的变化。
DOI：
10.1021/bi00354a034
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Shafritz,DA
中科院分区：
文献类型：
--
作者：
Panduro,A;Shalaby,F;Weiner,FR;Biempica,L;Zern,MA;Shafritz,DA
在大鼠CC 14诱导的肝再生过程中，白蛋白和甲胎蛋白基因的相对转录速率的变化与其他肝特异性和一般细胞功能基因一起被测量。在CC 14给药后24小时内，白蛋白基因转录降低85%，而甲胎蛋白转录从检测不到的水平增加到白蛋白观察到的50%。这些变化先于最大[3 H]胸苷掺入DNA，在48 h达到峰值。与肝脏特异性功能相关的其他基因，如配体蛋白、阿糖胞苷抗胰蛋白酶和细胞色素P-450，以及一般细胞基因pro α 1-和pro α 2-胶原蛋白，β-肌动蛋白和α-微管蛋白以动力学模式响应，通常彼此不同，并且与白蛋白和α-甲胎蛋白不同。白蛋白和甲胎蛋白mRNA稳态水平的变化与转录的变化相关，但甲胎蛋白mRNA的积累存在滞后，在CC 14给药后72 h达到峰值。这些研究表明，白蛋白和甲胎蛋白基因转录的相互变化发生在四氯化碳诱导的肝再生，导致这些特定的mRNA水平的变化。这些变化发生在DNA合成之前，似乎代表了肝细胞分化功能的改变以及肝再生过程。
During liver regeneration induced by CC14 administration to rats, changes in the relative transcription rates of albumin and a-fetoprotein genes have been measuredin conjunction with other liver-specific and general cellular function genes. Within 24 h following CC14 administration, albumin gene transcription decreases by 85%, whereas a-fetoprotein transcription increases from undetectable levels to 50% of that observed for albumin. These changes precede maximal [3H] thymidine incorporation into DNA which peaks at 48 h. Other genes related to liver-specific functions, such as ligandin, arantitrypsin, and cytochrome P-450’s, as well as general cellular genes pro a,-and pro a2-collagen,/?-actin, and a-tubulin, respond in kinetic patterns often distinct from each other and from albumin and a-fetoprotein. Changes in the steady-state levels of albumin and a-fetoprotein mRNA correlate with changes in transcription, but there is a lag in a-fetoprotein mRNA accumulation, which peaks at 72 h following CC14 administration. These studies indicate that reciprocal changes in albumin and a-fetoprotein gene transcription occur during CCl4-induced liver regeneration, leading to changes in the level of these specific mRNAs. These changes precede DNA synthesis and would appear to represent an alteration in differentiated function of hepatocytes in conjunction with the liver regenerative process.