Amino acid carbon isotope fingerprints are unique among eukaryotic microalgal taxonomic groups
Amino acid carbon isotope fingerprints are unique among eukaryotic microalgal taxonomic groups
复制标题
氨基酸碳同位素指纹在真核微藻分类群中是独特的
DOI:
10.1002/lno.12350
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发表时间:
2023
影响因子:
4.5
通讯作者:
McMahon, Kelton W.
中科院分区:
文献类型:
--
作者:
Stahl, Angela R.;Rynearson, Tatiana A.;McMahon, Kelton W.
Eukaryotic microalgae play critical roles in the structure and function of marine food webs. The contribution of microalgae to food webs can be tracked using compound‐specific isotope analysis of amino acids (CSIA‐AA). Previous CSIA‐AA studies have defined eukaryotic microalgae as a single functional group in food web mixing models, despite their vast taxonomic and ecological diversity. Using controlled cultures, this work characterizes the amino acidδ13C (δ13CAA) fingerprints—a multivariate metric of amino acid carbon isotope values—of four major groups of eukaryotic microalgae: diatoms, dinoflagellates, raphidophytes, and prasinophytes. We found excellent separation of essential amino acidδ13C (δ13CEAA) fingerprints among four microalgal groups (mean posterior probability reclassification of 99.2 ± 2.9%). We also quantified temperature effects, a primary driver of microalgal bulk carbon isotope variability, on the fidelity ofδ13CAAfingerprints. A 10°C range in temperature conditions did not have significant impacts on variance inδ13CAAvalues or the diagnostic microalgalδ13CEAAfingerprints. Theseδ13CEAAfingerprints were used to identify primary producers at the base of food webs supporting consumers in two contrasting systems: (1) penguins feeding in a diatom‐based food web and (2) mixotrophic corals receiving amino acids directly from autotrophic endosymbiotic dinoflagellates and indirectly from water column diatoms, prasinophytes, and cyanobacteria, likely via heterotrophic feeding on zooplankton. The increased taxonomic specificity of CSIA‐AA fingerprints developed here will greatly improve future efforts to reconstruct the contribution of diverse eukaryotic microalgae to the sources and cycling of organic matter in food web dynamics and biogeochemical cycling studies.
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影响因子:
1.3
作者:
Audrey G Rowe;K. Iken;A. Blanchard;D. O'Brien;Renate Døving Osvik;M. Uradnikova;M. Wooller
通讯作者:
M. Wooller
影响因子:
5.5
作者:
Besser, Alexi C.;Smith, Emma A. Elliott;Newsome, Seth D.
通讯作者:
Newsome, Seth D.
影响因子:
2.8
作者:
K. McMahon;Li Ling Hamady Simon R. Thorrold
通讯作者:
Li Ling Hamady Simon R. Thorrold
影响因子:
2.5
作者:
N. Phillips;E. Smith;S. Newsome;J. Houghton;Carol D. Carson;J. Alfaro‐Shigueto;J. Alfaro‐Shigueto;Jeffrey C. Mangel;Lawrence E. Eagling;L. Kubicek;Chris Harrod
通讯作者:
Chris Harrod
影响因子:
9.2
作者:
Fox, Michael D.;Williams, Gareth J.;Smith, Jennifer E.
通讯作者:
Smith, Jennifer E.