An intact mitochondrial cox1 gene and a pseudogene with different genomic configurations are present in apple cultivars 'Golden Delicious' and 'Delicious' : Evolutionary aspects
An intact mitochondrial cox1 gene and a pseudogene with different genomic configurations are present in apple cultivars 'Golden Delicious' and 'Delicious' : Evolutionary aspects
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苹果品种“金冠”和“美味”中存在完整的线粒体 cox1 基因和具有不同基因组配置的假基因：进化方面
DOI：
10.1016/j.scienta.2011.05.037
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发表时间：
2011
影响因子：
4.3
通讯作者：
A.Wakatsuki
中科院分区：
文献类型：
--
作者：
Tetsuo Yasugi;Umetsu Daiki;Satoshi Murakami;Makoto Sato;Tetsuya Tabata;A.Wakatsuki
我们对两个苹果品种“金冠”和“美味”中的线粒体 cox1 基因拷贝进行了表征。这两个品种都含有 cox1 的完整副本和截短的副本。完整的“Golden Delicious”和“Delicious”cox1 基因（分别命名为 G-cox1 和 D-cox1）被发现实际上都转录成约 1.7kb 的 RNA。两个完整的 cox1 和两个截短的副本（G-φcox1 和 D-φcox1）共享一个共同的 1115 bp 片段，两侧是两种不同 5'- 和 3'- 序列的四种组合。 PCR分析表明，携带G-cox1和G-φcox1的构型以亚化学计量的量存在于“美味”的线粒体基因组中，而携带D-φcox1的亚化学计量的分子存在于“金冠”线粒体基因组中。虽然不能推断 G-cox1 和 D-cox1 排列之间的祖先/后代关系，但结果使我们推测：(1) 包含祖代 cox1 部分的 1115-bp 片段被复制，从而生成伪 cox1 副本，(2) 随后在 1115-bp 重复序列的一部分上进行同源重组，从而产生后代 cox1 和伪 cox1 排列。
We have characterized the mitochondrial cox1 gene copies in two apple cultivars ‘Golden Delicious’ and ‘Delicious’. Both the cultivars contained an intact copy and a truncated copy of cox1. The intact ‘Golden Delicious’ and ‘Delicious’cox1 genes, designated G-cox1 and D-cox1, respectively, were both found to be actually transcribed to give an RNA of approximately 1.7kb. The two intact cox1 and two truncated copies (G-φcox1 and D-φcox1) shared a common 1115-bp segment flanked by four combinations of two different 5′- and 3′-sequences. PCR assay demonstrated that the configurations bearing G-cox1 and G-φcox1 existed in substoichiometric amounts within the mitochondrial genome of ‘Delicious’ whereas substoichiometric molecules carrying D-φcox1 were present in the ‘Golden Delicious’ mitochondrial genome. Although ancestor/descendant relationships cannot be inferred between the G-cox1 and D-cox1 arrangements, the results led us to hypothesize that (1) the 1115-bp segment containing part of the progenitor cox1 was duplicated, thereby generating a pseudo-cox1 copy, and (2) this was followed by homologous recombination across a portion of the 1115-bp repeats which gave rise to the descendant cox1 and pseudo-cox1 arrangements.