Characterization of a novel SGNH-type esterase from Lactobacillus plantarum.
Characterization of a novel SGNH-type esterase from Lactobacillus plantarum.
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DOI：
10.1016/j.ijbiomac.2016.12.061
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发表时间：
2017-03
影响因子：
8.2
通讯作者：
Yonggyu Kim;B. H. Ryu;Jimin Kim;Wanki Yoo;D. R. An;Boo-young Kim;Sena Kwon;Sojeong Lee;Ying Wang;Kyeong Kyu Kim;T. Doohun Kim
中科院分区：
文献类型：
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作者：
Yonggyu Kim;B. H. Ryu;Jimin Kim;Wanki Yoo;D. R. An;Boo-young Kim;Sena Kwon;Sojeong Lee;Ying Wang;Kyeong Kyu Kim;T. Doohun Kim
乳酸菌（LAB）是各种微生物酯水解酶的来源，因为它们可以产生广泛的短链酯，酚醇和脂肪酸。在这里，一种新的SGNH型酯酶（LpSGNH 1）从植物乳杆菌WCFS 1的鉴定，功能特征，并固定化生物技术应用。LpSGNH 1的同源物也在许多乳酸菌（LAB）物种中发现。利用质谱、凝胶过滤色谱、酶动力学、荧光和圆二色谱等方法对LpSGNH 1的生化特性进行了研究，结果表明LpSGNH 1在发酵过程中的各种条件下仍保持其活性。有趣的是，LpSGNH 1表现出广泛的底物，包括酮洛芬乙酸酯，头孢噻肟（CTX），和7-氨基头孢烷酸（7-ACA）以及葡萄糖五乙酸酯，乙酰木聚糖，和乙酰藻酸盐，这使得LpSGNH 1广泛的工业应用的一个伟大的候选人的能力。此外，与游离LpSGNH 1相比，交联酶聚集体LpSGNH 1（CLEA-LpSGNH 1）具有循环利用能力和热稳定性，可用于工业应用。本研究表明LpSGNH 1在制备商业化合物中的重要性，LpSGNH 1可作为乳酸菌SGNH酯酶的模型系统。
Lactic acid bacteria (LAB) are sources of a large variety of microbial ester hydrolases because they can produce a wide range of short-chain esters, phenolic alcohols, and fatty acids. Here, a novel SGNH-type esterase (LpSGNH1) fromLactobacillus plantarumWCFS1 was identified, functionally characterized, and immobilized for biotechnological applications. Homologs ofLpSGNH1 are also found in many lactic acid bacteria (LAB) species. Biochemical features ofLpSGNH1 were investigated using mass spectrometry, gel filtration chromatography, enzyme kinetics, fluorescence, and circular dichroism (CD) spectroscopy.LpSGNH1 were retained its activity under conditions that would be encountered during fermentations. Interestingly,LpSGNH1 exhibited the ability to act on a broad range of substrates including ketoprofen acetate, cefotaxime (CTX), and 7-aminocephalosporanic acid (7-ACA) as well as glucose pentaacetate, acetylxylan, and acetylalginate, which makeLpSGNH1 a great candidate for extensive industrial applications. Furthermore, cross-linked enzyme aggregates ofLpSGNH1 (CLEA-LpSGNH1) displayed recycling ability and thermal stability compared to freeLpSGNH1, which could be useful for industrial applications. This work highlights the importance ofLpSGNH1 in the preparation of commercial compounds, andLpSGNH1 can be used as a model system of SGNH esterases in lactic acid bacteria.