Down-regulated resistin level in consequence of decreased neutrophil counts in untreated Grave's disease.
Down-regulated resistin level in consequence of decreased neutrophil counts in untreated Grave's disease.
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未经治疗的格雷夫氏病中性粒细胞计数减少导致抵抗素水平下调
DOI：
10.18632/oncotarget.12019
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发表时间：
2016-11-29
期刊：
影响因子：
--
通讯作者：
Wang S
中科院分区：
文献类型：
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作者：
Peng Y;Qi Y;Huang F;Chen X;Zhou Y;Ye L;Wang W;Ning G;Wang S
抵抗素是一种富含半胱氨酸的分泌蛋白，主要由人体循环白细胞如中性粒细胞、单核细胞和巨噬细胞产生。迄今为止，很少有但有争议的研究报道了甲状腺功能亢进患者的抵抗素浓度，特别是Graves病（GD）。我们进行了一项对照前瞻性研究，探讨GD患者在-MMI治疗前后的血清抵抗素浓度。此外，我们还探讨了血清抵抗素水平的主要影响因素，并探讨了血清抵抗素在GD患者中的潜在作用。我们的研究纳入了39例未经治疗的GD （uGD）患者，其中男性8例，女性31例。除了25名健康对照外，所有这些患者都接受了MMI治疗。测量人体测量参数和激素评估。采用酶联免疫吸附法检测GD患者不同分期的血清抵抗素浓度。此外，通过对中性粒细胞NB4进行或不进行T3处理，检测甲状腺激素对抵抗素表达的影响。未经治疗的GD患者血清抵抗素水平和中性粒细胞计数明显下降。甲状腺GD （eGD）和trab阴性转化（nGD）患者经MMI治疗后，各项指标均恢复正常。抵抗素浓度与uGD患者FT3和FT4呈负相关，与中性粒细胞绝对数量呈正相关，而与甲状腺自身免疫抗体和BMI无相关性。中性粒细胞NB4在T3刺激下产生抵抗素表达降低。我们的研究显示GD患者血清抵抗素水平下降，我们认为血清抵抗素可能主要由循环中性粒细胞分泌，并在GD患者中因甲状腺激素过量而下调。
Resistin, belongs to cysteine-rich secretory protein, is mainly produced by circulating leukocytes, such as neutrophils monocytes and macrophages in humans. To date, few but controversial studies have reported about resistin concentrations in hyperthyroid patients, especially in Graves' disease (GD). We undertaked a controlled, prospective study to explore the serum resistin concentration in GD patients before and after -MMI treatment. In addition, we also investigated the main influencing factor on serum resistin level and discuessed the potential role of serum resistin plays in GD patients. 39 untreated GD (uGD) patients, including 8 males and 31 females, were enrolled in our investigation. All of these patients were prescribed with MMI treatment, in addition to 25 healthy controls. Anthropometric parameters and hormone assessment were measured. Enzyme-linked immunosorbent assay was used to detect serum resistin concentration in different stages of GD patients. Furthermore, neutrophil cell line NB4 with or without T3 treatment to detect the effect of thyroid hormones on resistin expression. The serum resistin level and neutrophil counts in untreated GD patients were significantly declined. And all of these parameters were recovered to normal after MMI treatment in ethyroid GD (eGD) and TRAb-negative conversion (nGD) patients. Resistin concentration exhibited a negative correlation with FT3 and FT4, but a positive correlation with absolute number of neutrophiles in uGD patients, whereas did not correlate with thyroid autoimmune antibodies and BMI. Neutrophile cell line, NB4, produced decreased expression of resistin when stimulated with T3. Our study showed a decrease of serum resistin level in GD patients and we suggested that the serum resistin might primarily secreted from circulating neutrophils and down-regulated by excessive thyroid hormones in GD patients.