4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway
4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway
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DOI：
10.5511/plantbiotechnology.21.1224b
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发表时间：
2022-03-01
影响因子：
1.6
通讯作者：
Sugimoto, Keiko
中科院分区：
文献类型：
--
作者：
Iwase, Akira;Takebayashi, Arika;Sugimoto, Keiko
4-苯基丁酸（4PBA）被用作治疗尿素循环障碍的药物，也被研究作为一种潜在的抗癌药物，通过其组蛋白去乙酰化酶（HDAC）抑制剂活性起作用。在寻找影响拟南芥植株再生的小分子的过程中，我们发现4PBA处理通过模拟外源生长素的作用来促进这一过程。具体而言，植物组织培养实验表明，含有4PBA的培养基可以促进愈伤组织的形成和随后的芽再生。对生长素应答或细胞分裂素应答标记系的分析表明，4PBA特异性地增强了生长素应答因子（ARF）依赖性的生长素应答。我们的western blot分析显示，与丁酸和trichostatin A等常用的HDAC抑制剂相比，4PBA处理并没有增强拟南芥的组蛋白乙酰化，这表明这种作用机制并不能解释4PBA对再生的影响。最后，质谱分析和遗传方法发现，拟南芥植物中的4PBA以独立于过氧化物酶体ibr3相关β -氧化的方式转化为苯乙酸（PAA），这是一种已知的天然生长素。该研究表明，4PBA通过其生长素活性促进外植体再生，不仅在植物组织培养工程中具有潜在的应用价值，而且在植物β -氧化途径的研究中也具有潜在的应用价值。
4-Phenylbutyric acid (4PBA) is utilized as a drug to treat urea cycle disorders and is also being studied as a potential anticancer drug that acts via its histone deacetylase (HDAC) inhibitor activity. During a search to find small molecules that affect plant regeneration in Arabidopsis, we found that 4PBA treatment promotes this process by mimicking the effect of exogenous auxin. Specifically, plant tissue culture experiments revealed that a medium containing 4PBA enhances callus formation and subsequent shoot regeneration. Analyses with auxin-responsive or cytokinin-responsive marker lines demonstrated that 4PBA specifically enhances AUXIN RESPONSE FACTOR (ARF)-dependent auxin responses. Our western blot analyses showed that 4PBA treatment does not enhance histone acetylation in Arabidopsis, in contrast to butyric acid and trichostatin A, other chemicals often used as HDAC inhibitors, suggesting this mechanism of action does not explain the observed effect of 4PBA on regeneration. Finally, mass spectroscopic analysis and genetic approaches uncovered that 4PBA in Arabidopsis plants is converted to phenylacetic acid (PAA), a known natural auxin, in a manner independent of peroxisomal IBR3-related beta-oxidation. This study demonstrates that 4PBA application promotes regeneration in explants via its auxin activity and has potential applications to not only plant tissue culture engineering but also research on the plant beta-oxidation pathway.