Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields
Recent Insights into the Crystallization Process; Protein Crystal Nucleation and Growth Peculiarities; Processes in the Presence of Electric Fields
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DOI：
10.3390/cryst7100310
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发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
C. Nanev
中科院分区：
文献类型：
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作者：
C. Nanev
三维蛋白质分子结构对于获得对人类基因组的更深入了解和开发新的基于蛋白质的药物至关重要。对这种结构的X射线衍射研究需要衍射良好的蛋白质晶体。一组外部物理因素可以促进和指导蛋白质结晶，使得获得的晶体可用于X射线研究。电场的应用有助于控制蛋白质晶体尺寸和衍射质量。综述了电场作用下蛋白质晶体的成核与生长。介观水平的影响蛋白质结晶行使的电场的概念也被认为是。
Three-dimensional protein molecule structures are essential for acquiring a deeper insight of the human genome, and for developing novel protein-based pharmaceuticals. X-ray diffraction studies of such structures require well-diffracting protein crystals. A set of external physical factors may promote and direct protein crystallization so that crystals obtained are useful for X-ray studies. Application of electric fields aids control over protein crystal size and diffraction quality. Protein crystal nucleation and growth in the presence of electric fields are reviewed. A notion of mesoscopic level of impact on the protein crystallization exercised by an electric field is also considered.