Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
Non-viral derivation of a transgene-free induced pluripotent stem cell line from a male beagle dog
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来自雄性比格犬的无转基因诱导多能干细胞系的非病毒衍生
DOI：
10.1016/j.scr.2021.102375
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发表时间：
2021
影响因子：
1.2
通讯作者：
Okano Hideyuki
中科院分区：
文献类型：
--
作者：
Yoshimatsu Sho;Edamura Kazuya;Yoshii Yumi;Iguchi Aozora;Kondo Hirotaka;Shibuya Hisashi;Sato Tsukika;Shiozawa Seiji;Okano Hideyuki
我们先前报道了使用优化的诱导培养基和无整合的附加型载体从雌性比格犬的体细胞成纤维细胞非病毒衍生无转基因诱导多能干细胞（iPSC）。在这里，我们报告了雄性犬iPSC系OF 35 Y-iPS的新衍生物，其显示出多能性的标准特征，例如多能性标记物的强基因表达谱、分化成所有三个胚层的潜力和正常核型（78 XY）。此外，我们证明了2A-EGFP靶向整合到犬NANOS 3基因座中。新的iPSC系将成为干细胞研究和再生兽医学的有用资源。
We previously reported the non-viral derivation of transgene-free induced pluripotent stem cells (iPSCs) from somatic fibroblasts of a female beagle dog using an optimized induction medium and integration-free episomal vectors. Here, we report novel derivation of a male canine iPSC line OF35Y-iPS, which showed standard characteristics of pluripotency such as a strong gene expression profile of pluripotency markers, differentiation potential into all three germ layers, and normal karyotype (78XY). Furthermore, we demonstrated targeted integration of 2A-EGFP into the canineNANOS3locus. The novel iPSC line would be a useful resource for stem cell research and regenerative veterinary medicine.